BMRB Entry 26700

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26700

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Title:
Backbone assignment of Sam68 STAR domain
Deposition date:
2015-11-04
Original release date:
2016-01-06
Authors:
Foot, Jaelle; Dominguez, Cyril
Citation:

Citation: Feracci, Mikael; Foot, Jaelle; Grellscheid, Sushma; Danilenko, Marina; Stehle, Ralf; Gonchar, Oksana; Kang, Hyun-Seo; Dalgliesh, Caroline; Meyer, Helge; Liu, Yilei; Lahat, Albert; Sattler, Michael; Eperon, Ian; Elliott, David; Dominguez, Cyril. "Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68"  Nat. Commun. 7, 10355-10355 (2016).
PubMed: 26758068

Assembly members:

Assembly members:
Sam68, polymer, 189 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLEICS-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sam68: GAMEPENKYLPELMAEKDSL DPSFTHAMQLLTAEIEKIQK GDSKKDDEENYLDLFSHKNM KLKERVLIPVKQYPKFNFVG KILGPQGNTIKRLQEETGAK ISVLGKGSMRDKAKEEELRK GGDPKYAHLNMDLHVFIEVF GPPCEAYALMAHAMEEVKKF LVPDMMDDICQEQFLELSYL NGVPEPSRG

Data sets:
Data typeCount
15N chemical shifts144
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sam681

Entities:

Entity 1, Sam68 189 residues - Formula weight is not available

residues 1 and 2 are non-native amino acids from the tag after Tev cleavage

1   GLYALAMETGLUPROGLUASNLYSTYRLEU
2   PROGLULEUMETALAGLULYSASPSERLEU
3   ASPPROSERPHETHRHISALAMETGLNLEU
4   LEUTHRALAGLUILEGLULYSILEGLNLYS
5   GLYASPSERLYSLYSASPASPGLUGLUASN
6   TYRLEUASPLEUPHESERHISLYSASNMET
7   LYSLEULYSGLUARGVALLEUILEPROVAL
8   LYSGLNTYRPROLYSPHEASNPHEVALGLY
9   LYSILELEUGLYPROGLNGLYASNTHRILE
10   LYSARGLEUGLNGLUGLUTHRGLYALALYS
11   ILESERVALLEUGLYLYSGLYSERMETARG
12   ASPLYSALALYSGLUGLUGLULEUARGLYS
13   GLYGLYASPPROLYSTYRALAHISLEUASN
14   METASPLEUHISVALPHEILEGLUVALPHE
15   GLYPROPROCYSGLUALATYRALALEUMET
16   ALAHISALAMETGLUGLUVALLYSLYSPHE
17   LEUVALPROASPMETMETASPASPILECYS
18   GLNGLUGLNPHELEUGLULEUSERTYRLEU
19   ASNGLYVALPROGLUPROSERARGGLY

Related Database Links:

UNP Q07666
AlphaFold Q99760

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks