BMRB Entry 26819

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26819

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Title:
1H, 13C and 15N backbone and side chain chemical shift assignments of CHIP-TPR
Deposition date:
2016-06-17
Original release date:
2016-10-13
Authors:
Zhang, Huaqun; Page, Richard
Citation:

Citation: Zhang, Huaqun; McGlone, Cameron; Mannion, Matthew; Page, Richard. "1H, 15N and 13C resonance assignments for free and IEEVD peptide-bound forms of the tetratricopeptide repeat domain from the human E3 ubiquitin ligase CHIP"  Biomol. NMR Assign. 11, 5-9 (2016).
PubMed: 27709416

Assembly members:

Assembly members:
CHIP-TPR, polymer, 139 residues, 15860 Da.
Hsp70p1, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHis//2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CHIP-TPR: GAMGSEKSPSAQELKEQGNR LFVGRKYPEAAACYGRAITR NPLVAVYYTNRALCYLKMQQ HEQALADCRRALELDGQSVK AHFFLGQCQLEMESYDEAIA NLQRAYSLAKEQRLNFGDDI PSALRIAKKKRWNSIEERR
Hsp70p1: GPTIEEVD

Data sets:
Data typeCount
13C chemical shifts362
15N chemical shifts121
1H chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CHIP-TPR1
2Hsp70p12

Entities:

Entity 1, CHIP-TPR 139 residues - 15860 Da.

Residues 1-5 are from a non-native affinity tag after cleavage by TEV protease.

1   GLYALAMETGLYSERGLULYSSERPROSER
2   ALAGLNGLULEULYSGLUGLNGLYASNARG
3   LEUPHEVALGLYARGLYSTYRPROGLUALA
4   ALAALACYSTYRGLYARGALAILETHRARG
5   ASNPROLEUVALALAVALTYRTYRTHRASN
6   ARGALALEUCYSTYRLEULYSMETGLNGLN
7   HISGLUGLNALALEUALAASPCYSARGARG
8   ALALEUGLULEUASPGLYGLNSERVALLYS
9   ALAHISPHEPHELEUGLYGLNCYSGLNLEU
10   GLUMETGLUSERTYRASPGLUALAILEALA
11   ASNLEUGLNARGALATYRSERLEUALALYS
12   GLUGLNARGLEUASNPHEGLYASPASPILE
13   PROSERALALEUARGILEALALYSLYSLYS
14   ARGTRPASNSERILEGLUGLUARGARG

Entity 2, Hsp70p1 8 residues - Formula weight is not available

1   GLYPROTHRILEGLUGLUVALASP

Related Database Links:

UNP Q9UNE7 P0DMV8
AlphaFold Q9HBT1 Q9UQM0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks