BMRB Entry 26907

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26907

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Title:
Chemical shift assignments of a hydrophobin from Phanerochaete carnosa
Deposition date:
2016-09-23
Original release date:
2017-04-26
Authors:
Langelaan, David; Smith, Steven
Citation:

Citation: Gandier, Julie-Anne; Langelaan, David; Won, Amy; O'Donnell, Kylie; Grondin, Julie; Spencer, Holly; Wong, Philip; Tillier, Elisabeth; Yip, Christopher; Smith, Steven; Master, Emma. "Characterization of a Basidiomycota hydrophobin reveals the structural basis for a high-similarity Class I subdivision"  Sci. Rep. 7, 45863-45863 (2017).
PubMed: 28393921

Assembly members:

Assembly members:
hfb4, polymer, 126 residues, 12301.7867 Da.

Natural source:

Natural source:   Common Name: Phanerochaete carnosa   Taxonomy ID: 231932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Phanerochaete carnosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hfb4: KAMADIGSTPNPENAKRWAT TTTPQAPTTTVTVTETATAS SVPASDCSTGSVQCCNTVTS ASDPTAAGILGLLGVVVQGV DVLVGLTCDPITVVGAGGGS CSAQAVCCQDNSYGSLISIG CIPVTL

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts79
1H chemical shifts475

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1hfb41

Entities:

Entity 1, hfb4 126 residues - 12301.7867 Da.

1   LYSALAMETALAASPILEGLYSERTHRPRO
2   ASNPROGLUASNALALYSARGTRPALATHR
3   THRTHRTHRPROGLNALAPROTHRTHRTHR
4   VALTHRVALTHRGLUTHRALATHRALASER
5   SERVALPROALASERASPCYSSERTHRGLY
6   SERVALGLNCYSCYSASNTHRVALTHRSER
7   ALASERASPPROTHRALAALAGLYILELEU
8   GLYLEULEUGLYVALVALVALGLNGLYVAL
9   ASPVALLEUVALGLYLEUTHRCYSASPPRO
10   ILETHRVALVALGLYALAGLYGLYGLYSER
11   CYSSERALAGLNALAVALCYSCYSGLNASP
12   ASNSERTYRGLYSERLEUILESERILEGLY
13   CYSILEPROVALTHRLEU

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