BMRB Entry 26942

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26942

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Title:
Backbone and Side-Chain (1)H, (13)C, and (15)N Chemical Shift Assignments for the homodimeric Histone-like DNA binding protein (Hup) of Helicobacter pylori
Deposition date:
2016-11-12
Original release date:
2018-03-16
Authors:
Jaiswal, Nancy; Raikwal, Nisha; Pandey, Himanshu; Arora, Ashish; Kumar, Dinesh
Citation:

Citation: Jaiswal, Nancy; Raikwal, Nisha; Pandey, Himanshu; Agarwal, Nipanshu; Arora, Ashish; Poluri, Krishna Mohan; Kumar, Dinesh. "NMR elucidation of monomer-dimer transition and conformational heterogeneity in histone-like DNA binding protein of Helicobacter pylori"  Magn. Reson. Chem. 56, 285-299 (2018).
PubMed: 29241299

Assembly members:

Assembly members:
Hup, polymer, 98 residues, 10772 Da.

Natural source:

Natural source:   Common Name: Helicobacter pylori   Taxonomy ID: 210   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter pylori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE80L

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hup: GAMEMNKAEFIDLVKEAGKY NSKREAEEAISAFTLAVETA LSKGESVELIGFGKFETAEQ KGKEGKVPGSDKTYKTEDKR VPKFKPGKTLKQKVEEGK

Data sets:
Data typeCount
13C chemical shifts570
15N chemical shifts170
1H chemical shifts421

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Hup, Monomer 11
2Hup, Monomer 21

Entities:

Entity 1, Hup, Monomer 1 98 residues - 10772 Da.

Residue -4 to -1 represent cloning artifacts

1   GLYALAMETGLUMETASNLYSALAGLUPHE
2   ILEASPLEUVALLYSGLUALAGLYLYSTYR
3   ASNSERLYSARGGLUALAGLUGLUALAILE
4   SERALAPHETHRLEUALAVALGLUTHRALA
5   LEUSERLYSGLYGLUSERVALGLULEUILE
6   GLYPHEGLYLYSPHEGLUTHRALAGLUGLN
7   LYSGLYLYSGLUGLYLYSVALPROGLYSER
8   ASPLYSTHRTYRLYSTHRGLUASPLYSARG
9   VALPROLYSPHELYSPROGLYLYSTHRLEU
10   LYSGLNLYSVALGLUGLUGLYLYS

Related Database Links:

NCBI WP_001029082.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks