BMRB Entry 26945

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26945

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Title:
Human SMAD4 MH1 domain
Deposition date:
2016-11-17
Original release date:
2017-11-06
Authors:
Macias, Maria J.; Batet, Marta
Citation:

Citation: Martin-Malpartida, Pau; Batet, Marta; Kaczmarska, Zuzanna; Freier, Regina; Gomes, Tiago; Aragon, Eric; Zou, Yilong; Wang, Qiong; Xi, Qiaoran; Ruiz, Lidia; Vea, Angela; Marquez, Jose; Massague, Joan; Macias, Maria. "Structural basis for genome wide recognition of 5-bp GC motifs by SMAD transcription factors"  Nat. Commun. 8, 2070-2070 (2017).
PubMed: 29234012

Assembly members:

Assembly members:
SMAD4, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: petM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SMAD4: GAMGPTSNDACLSIVHSLMC HRQGGESETFAKRAIESLVK KLKEKKDELDSLITAITTNG AHPSKCVTIQRTLDGRLQVA GRKGFPHVIYARLWRWPDLH KNELKHVKYCQYAFDLKCDS VCVNPYHYERVVSPG

Data sets:
Data typeCount
13C chemical shifts233
15N chemical shifts109
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SMAD41

Entities:

Entity 1, SMAD4 135 residues - Formula weight is not available

Residues 6-9 are artifacts from the cloning site

1   GLYALAMETGLYPROTHRSERASNASPALA
2   CYSLEUSERILEVALHISSERLEUMETCYS
3   HISARGGLNGLYGLYGLUSERGLUTHRPHE
4   ALALYSARGALAILEGLUSERLEUVALLYS
5   LYSLEULYSGLULYSLYSASPGLULEUASP
6   SERLEUILETHRALAILETHRTHRASNGLY
7   ALAHISPROSERLYSCYSVALTHRILEGLN
8   ARGTHRLEUASPGLYARGLEUGLNVALALA
9   GLYARGLYSGLYPHEPROHISVALILETYR
10   ALAARGLEUTRPARGTRPPROASPLEUHIS
11   LYSASNGLULEULYSHISVALLYSTYRCYS
12   GLNTYRALAPHEASPLEULYSCYSASPSER
13   VALCYSVALASNPROTYRHISTYRGLUARG
14   VALVALSERPROGLY

Related Database Links:

EMBL ENST00000342988.7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks