BMRB Entry 26958

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26958

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Title:
Backbone assignment of mouse prion (23-231) at pH 4 and 37C
Deposition date:
2016-11-25
Original release date:
2017-08-10
Authors:
Sengupta, Ishita; Bhate, Suhas; Udgaonkar, Jayant; Das, Ranabir
Citation:

Citation: Sengupta, Ishita; Bhate, Suhas; Das, Ranabir; Udgaonkar, Jayant. "Salt-Mediated Oligomerization of the Mouse Prion Protein Monitored by Real-Time NMR"  J. Mol. Biol. 429, 1852-1872 (2017).
PubMed: 28502793

Assembly members:

Assembly members:
MoPrp, polymer, 211 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MoPrp: MKKRPKPGGWNTGGSRYPGQ GSPGGNRYPPQGGTWGQPHG GGWGQPHGGSWGQPHGGSWG QPHGGGWGQGGGTHNQWNKP SKPKTNLKHVAGAAAAGAVV GGLGGYMLGSAMSRPMIHFG NDWEDRYYRENMYRYPNQVY YRPVDQYSNQNNFVHDCVNI TIKQHTVTTTTKGENFTETD VKMMERVVEQMCVTQYQKES QAYYDGRRSSS

Data sets:
Data typeCount
13C chemical shifts532
15N chemical shifts183
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Prion monomer1

Entities:

Entity 1, Prion monomer 211 residues - Formula weight is not available

1   METLYSLYSARGPROLYSPROGLYGLYTRP
2   ASNTHRGLYGLYSERARGTYRPROGLYGLN
3   GLYSERPROGLYGLYASNARGTYRPROPRO
4   GLNGLYGLYTHRTRPGLYGLNPROHISGLY
5   GLYGLYTRPGLYGLNPROHISGLYGLYSER
6   TRPGLYGLNPROHISGLYGLYSERTRPGLY
7   GLNPROHISGLYGLYGLYTRPGLYGLNGLY
8   GLYGLYTHRHISASNGLNTRPASNLYSPRO
9   SERLYSPROLYSTHRASNLEULYSHISVAL
10   ALAGLYALAALAALAALAGLYALAVALVAL
11   GLYGLYLEUGLYGLYTYRMETLEUGLYSER
12   ALAMETSERARGPROMETILEHISPHEGLY
13   ASNASPTRPGLUASPARGTYRTYRARGGLU
14   ASNMETTYRARGTYRPROASNGLNVALTYR
15   TYRARGPROVALASPGLNTYRSERASNGLN
16   ASNASNPHEVALHISASPCYSVALASNILE
17   THRILELYSGLNHISTHRVALTHRTHRTHR
18   THRLYSGLYGLUASNPHETHRGLUTHRASP
19   VALLYSMETMETGLUARGVALVALGLUGLN
20   METCYSVALTHRGLNTYRGLNLYSGLUSER
21   GLNALATYRTYRASPGLYARGARGSERSER
22   SER

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks