BMRB Entry 27022

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27022

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Title:
1H, 15N, 13C backbone resonance assignments of human phosphoglycerate kinase in a transition state analogue complex with ADP, 3-phosphoglycerate and magnesium trifluoride
Deposition date:
2017-02-03
Original release date:
2017-09-06
Authors:
Serimbetov, Zhalgas; Baxter, Nicola
Citation:

Citation: Serimbetov, Zhalgas; Baxter, Nicola; Cliff, Matthew; Waltho, Jonathan. "1H, 15N, 13C backbone resonance assignments of human phosphoglycerate kinase in a transition state analogue complex with ADP, 3-phosphoglycerate and magnesium trifluoride"  Biomol. NMR Assignments 11, 251-256 (2017).
PubMed: 28866776

Assembly members:

Assembly members:
PGK, polymer, 417 residues, 44614.69 Da.
entity_ADP, non-polymer, 427.201 Da.
entity_3PG, non-polymer, 186.057 Da.
entity_MGF, non-polymer, 81.300 Da.
entity_MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PGK: MSLSNKLTLDKLDVKGKRVV MRVDFNVPMKNNQITNNQRI KAAVPSIKFCLDNGAKSVVL MSHLGRPDGVPMPDKYSLEP VAVELKSLLGKDVLFLKDCV GPEVEKACANPAAGSVILLE NLRFHVEEEGKGKDASGNKV KAEPAKIEAFRASLSKLGDV YVNDAFGTAHRAHSSMVGVN LPQKAGGFLMKKELNYFAKA LESPERPFLAILGGAKVADK IQLINNMLDKVNEMIIGGGM AFTFLKVLNNMEIGTSLFDE EGAKIVKDLMSKAEKNGVKI TLPVDFVTADKFDENAKTGQ ATVASGIPAGWMGLDCGPES SKKYAEAVTRAKQIVWNGPV GVFEWEAFARGTKALMDEVV KATSRGCITIIGGGDTATCC AKWNTEDKVSHVSTGGGASL ELLEGKVLPGVDALSNI

Data sets:
Data typeCount
13C chemical shifts1186
15N chemical shifts385
1H chemical shifts385

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PGK1
2ADP2
33PG3
4MgF3-4
5Mg2+5

Entities:

Entity 1, PGK 417 residues - 44614.69 Da.

1   METSERLEUSERASNLYSLEUTHRLEUASP
2   LYSLEUASPVALLYSGLYLYSARGVALVAL
3   METARGVALASPPHEASNVALPROMETLYS
4   ASNASNGLNILETHRASNASNGLNARGILE
5   LYSALAALAVALPROSERILELYSPHECYS
6   LEUASPASNGLYALALYSSERVALVALLEU
7   METSERHISLEUGLYARGPROASPGLYVAL
8   PROMETPROASPLYSTYRSERLEUGLUPRO
9   VALALAVALGLULEULYSSERLEULEUGLY
10   LYSASPVALLEUPHELEULYSASPCYSVAL
11   GLYPROGLUVALGLULYSALACYSALAASN
12   PROALAALAGLYSERVALILELEULEUGLU
13   ASNLEUARGPHEHISVALGLUGLUGLUGLY
14   LYSGLYLYSASPALASERGLYASNLYSVAL
15   LYSALAGLUPROALALYSILEGLUALAPHE
16   ARGALASERLEUSERLYSLEUGLYASPVAL
17   TYRVALASNASPALAPHEGLYTHRALAHIS
18   ARGALAHISSERSERMETVALGLYVALASN
19   LEUPROGLNLYSALAGLYGLYPHELEUMET
20   LYSLYSGLULEUASNTYRPHEALALYSALA
21   LEUGLUSERPROGLUARGPROPHELEUALA
22   ILELEUGLYGLYALALYSVALALAASPLYS
23   ILEGLNLEUILEASNASNMETLEUASPLYS
24   VALASNGLUMETILEILEGLYGLYGLYMET
25   ALAPHETHRPHELEULYSVALLEUASNASN
26   METGLUILEGLYTHRSERLEUPHEASPGLU
27   GLUGLYALALYSILEVALLYSASPLEUMET
28   SERLYSALAGLULYSASNGLYVALLYSILE
29   THRLEUPROVALASPPHEVALTHRALAASP
30   LYSPHEASPGLUASNALALYSTHRGLYGLN
31   ALATHRVALALASERGLYILEPROALAGLY
32   TRPMETGLYLEUASPCYSGLYPROGLUSER
33   SERLYSLYSTYRALAGLUALAVALTHRARG
34   ALALYSGLNILEVALTRPASNGLYPROVAL
35   GLYVALPHEGLUTRPGLUALAPHEALAARG
36   GLYTHRLYSALALEUMETASPGLUVALVAL
37   LYSALATHRSERARGGLYCYSILETHRILE
38   ILEGLYGLYGLYASPTHRALATHRCYSCYS
39   ALALYSTRPASNTHRGLUASPLYSVALSER
40   HISVALSERTHRGLYGLYGLYALASERLEU
41   GLULEULEUGLUGLYLYSVALLEUPROGLY
42   VALASPALALEUSERASNILE

Entity 2, ADP - C10 H15 N5 O10 P2 - 427.201 Da.

1   ADP

Entity 3, 3PG - C3 H7 O7 P - 186.057 Da.

1   3PG

Entity 4, MgF3- - F3 Mg - 81.300 Da.

1   MGF

Entity 5, Mg2+ - Mg - 24.305 Da.

1   MG

Related Database Links:

PDB 2WZB 2XE6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks