BMRB Entry 27030

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27030

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Title:
Chemical shift assignments of DANCER-0, a dynamic pentamutant of the B1 domain of streptococcal protein G (GB1)
Deposition date:
2017-02-10
Original release date:
2017-10-10
Authors:
Damry, Adam; Davey, James; Goto, Natalie; Chica, Roberto
Citation:

Citation: Davey, James; Damry, Adam; Goto, Natalie; Chica, Roberto. "Rational design of proteins that exchange on functional timescales."  Nat. Chem. Biol. 13, 1280-1285 (2017).
PubMed: 29058725

Assembly members:

Assembly members:
DANCER-0, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptococcus sp. GX7805   Taxonomy ID: 1325   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus Streptococcus sp. GX7805

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ414

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DANCER-0: MHHHHHHGMTFKAIINGKTL KGETTTEAVDAATAEKVFKQ YFNDNGIDGEWTYDDATKTF TVTE

Data sets:
Data typeCount
13C chemical shifts203
15N chemical shifts47
1H chemical shifts305

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1DANCER-01

Entities:

Entity 1, DANCER-0 64 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLYMETTHR
2   PHELYSALAILEILEASNGLYLYSTHRLEU
3   LYSGLYGLUTHRTHRTHRGLUALAVALASP
4   ALAALATHRALAGLULYSVALPHELYSGLN
5   TYRPHEASNASPASNGLYILEASPGLYGLU
6   TRPTHRTYRASPASPALATHRLYSTHRPHE
7   THRVALTHRGLU

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