BMRB Entry 27035

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27035

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Title:
Backbone assignment of human Ube2T
Deposition date:
2017-02-14
Original release date:
2017-04-25
Authors:
Bortoluzzi, Alessio; Morreale, Francesca; Walden, Helen; Ciulli, Alessio
Citation:

Citation: Morreale, Francesca; Bortoluzzi, Alessio; Chaugule, Viduth; Arkinson, Connor; Walden, Helen; Ciulli, Alessio. "Allosteric targeting of the Fanconi anemia ubiquitin-conjugating enzyme Ube2T by fragment screening"  J. Med. Chem. 60, 4093-4098 (2017).
PubMed: 28437106

Assembly members:

Assembly members:
Ube2T_(1-154), polymer, 154 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ube2T_(1-154): MQRASRLKRELHMLATEPPP GITCWQDKDQMDDLRAQILG GANTPYEKGVFKLEVIIPER YPFEPPQIRFLTPIYHPNID SAGRICLDVLKLPPKGAWRP SLNIATVLTSIQLLMSEPNP DDPLMADISSEFKYNKPAFL KNARQWTEKHARQK

Data sets:
Data typeCount
13C chemical shifts399
15N chemical shifts126
1H chemical shifts126

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ube2T (1-154)1

Entities:

Entity 1, Ube2T (1-154) 154 residues - Formula weight is not available

1   METGLNARGALASERARGLEULYSARGGLU
2   LEUHISMETLEUALATHRGLUPROPROPRO
3   GLYILETHRCYSTRPGLNASPLYSASPGLN
4   METASPASPLEUARGALAGLNILELEUGLY
5   GLYALAASNTHRPROTYRGLULYSGLYVAL
6   PHELYSLEUGLUVALILEILEPROGLUARG
7   TYRPROPHEGLUPROPROGLNILEARGPHE
8   LEUTHRPROILETYRHISPROASNILEASP
9   SERALAGLYARGILECYSLEUASPVALLEU
10   LYSLEUPROPROLYSGLYALATRPARGPRO
11   SERLEUASNILEALATHRVALLEUTHRSER
12   ILEGLNLEULEUMETSERGLUPROASNPRO
13   ASPASPPROLEUMETALAASPILESERSER
14   GLUPHELYSTYRASNLYSPROALAPHELEU
15   LYSASNALAARGGLNTRPTHRGLULYSHIS
16   ALAARGGLNLYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks