BMRB Entry 27051

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PDB ID: 6nan
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27051
MolProbity Validation Chart

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Title:
1H, 15N and 13C resonance assignments of the Ixolaris, a tissue factor pathway inhibitor from the tick salivary gland
Deposition date:
2017-03-19
Original release date:
2019-01-18
Authors:
de Paula, Viviane; Silva, Felipe; Francischette, Ivo; Monteiro, Robson; Valente, Ana Paula
Citation:

Citation: de Paula, Viviane; Silva, Felipe; Francischette, Ivo; Monteiro, Robson; Valente, Ana Paula. "1H, 15N and 13C resonance assignments of Ixolaris, a tissue factor pathway inhibitor from the tick salivary gland"  Biomol. NMR Assign. 11, 293-296 (2017).
PubMed: 28856584

Assembly members:

Assembly members:
Ixolaris, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: black-legged tick   Taxonomy ID: 6945   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ixodes scapularis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ixolaris: AMAERVSEMDIYEFESWVSC LDPEQVTCESQEGTHASYNR KTGQCEEQKGTECGGGENHF ETLLKCNESCNDAPKPPCSL EVDYGVGRANIPRWYYDTNN ATCEMFTYGGITGNKNNFES EEECKETCKGFSLLKKVNVT IN

Data sets:
Data typeCount
13C chemical shifts515
15N chemical shifts134
1H chemical shifts828

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ixolaris1

Entities:

Entity 1, Ixolaris 142 residues - Formula weight is not available

Residues 1-2 (A,M) represent a non-native affinity tag

1   ALAMETALAGLUARGVALSERGLUMETASP
2   ILETYRGLUPHEGLUSERTRPVALSERCYS
3   LEUASPPROGLUGLNVALTHRCYSGLUSER
4   GLNGLUGLYTHRHISALASERTYRASNARG
5   LYSTHRGLYGLNCYSGLUGLUGLNLYSGLY
6   THRGLUCYSGLYGLYGLYGLUASNHISPHE
7   GLUTHRLEULEULYSCYSASNGLUSERCYS
8   ASNASPALAPROLYSPROPROCYSSERLEU
9   GLUVALASPTYRGLYVALGLYARGALAASN
10   ILEPROARGTRPTYRTYRASPTHRASNASN
11   ALATHRCYSGLUMETPHETHRTYRGLYGLY
12   ILETHRGLYASNLYSASNASNPHEGLUSER
13   GLUGLUGLUCYSLYSGLUTHRCYSLYSGLY
14   PHESERLEULEULYSLYSVALASNVALTHR
15   ILEASN

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