BMRB Entry 27083

Title:
NMR backbone assignment of the Tyrosine Kinase domain of human fibroblast receptor 3 in apo state and in complex with inhibitor PD173074
Deposition date:
2017-04-24
Original release date:
2018-03-15
Authors:
Sanfelice, Domenico; Koss, Hans; Bunney, Tom; Katan, Matilda
Citation:

Citation: Bunney, Tom; Inglis, Alison; Sanfelice, Domenico; Farrell, Brendan; Kerr, Christopher; Thompson, Gary; Masson, Glenn; Thiyagarajan, Nethaji; Svergun, Dmitri; Williams, Roger; Breeze, Alexander; Katan, Matilda. "Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System"  Structure 26, 446-458 (2018).
PubMed: 29478821

Assembly members:

Assembly members:
FGFR3, polymer, 314 residues, Formula weight is not available
1-TERT-BUTYL-3-[6-(3,5-DIMETHOXY-PHENYL)-2-(4-DIETHYLAMINO-BUTYLAMINO)-PYRIDO[2,3-D]PYRIMIDIN-7-YL]-UREA, non-polymer, 523.670 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pOPINS

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts231
1H chemical shifts227

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Holo FGFR31
2PD1730742

Entities:

Entity 1, Holo FGFR3 314 residues - Formula weight is not available

1   SERGLULEUGLULEUPROALAASPPROLYS
2   TRPGLULEUSERARGALAARGLEUTHRLEU
3   GLYLYSPROLEUGLYGLUGLYALAPHEGLY
4   GLNVALVALMETALAGLUALAILEGLYILE
5   ASPLYSASPARGALAALALYSPROVALTHR
6   VALALAVALLYSMETLEULYSASPASPALA
7   THRASPLYSASPLEUSERASPLEUVALSER
8   GLUMETGLUMETMETLYSMETILEGLYLYS
9   HISLYSASNILEILEASNLEULEUGLYALA
10   CYSTHRGLNGLYGLYPROLEUTYRVALLEU
11   VALGLUTYRALAALALYSGLYASNLEUARG
12   GLUPHELEUARGALAARGARGPROPROGLY
13   LEUASPTYRSERPHEASPTHRSERLYSPRO
14   PROGLUGLUGLNLEUTHRPHELYSASPLEU
15   VALSERCYSALATYRGLNVALALAARGGLY
16   METGLUTYRLEUALASERGLNLYSCYSILE
17   HISARGASPLEUALAALAARGASNVALLEU
18   VALTHRGLUASPASNVALMETLYSILEALA
19   ASPPHEGLYLEUALAARGASPVALHISASN
20   LEUASPTYRTYRLYSLYSTHRTHRASNGLY
21   ARGLEUPROVALLYSTRPMETALAPROGLU
22   ALALEUPHEASPARGVALTYRTHRHISGLN
23   SERASPVALTRPSERPHEGLYVALLEULEU
24   TRPGLUILEPHETHRLEUGLYGLYSERPRO
25   TYRPROGLYILEPROVALGLUGLULEUPHE
26   LYSLEULEULYSGLUGLYHISARGMETASP
27   LYSPROALAASNCYSTHRHISASPLEUTYR
28   METILEMETARGGLUCYSTRPHISALAALA
29   PROSERGLNARGPROTHRPHELYSGLNLEU
30   VALGLUASPLEUASPARGVALLEUTHRVAL
31   THRSERTHRASPGLUTYRLEUASPLEUSER
32   ALAPROPHEGLU

Entity 2, PD173074 - C28 H41 N7 O3 - 523.670 Da.

1   PD1

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