BMRB Entry 27090

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27090

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Elongation factor P
Deposition date:
2017-05-02
Original release date:
2017-10-03
Authors:
Macosek, Jakub; Hennig, Janosch
Citation:

Citation: Krafczyk, Ralph; Macosek, Jakub; Jagtap, Pravin Kumar Ankush; Gast, Daniel; Wunder, Swetlana; Mitra, Prithiba; Jha, Amit Kumar; Rohr, Jurgen; Hoffmann-Roder, Anja; Jung, Kirsten; Hennig, Janosch; Lassak, Jurgen. "Structural Basis for EarP-Mediated Arginine Glycosylation of Translation Elongation Factor EF-P"  MBio 8, e01412-e01417 (2017).
PubMed: 28951478

Assembly members:

Assembly members:
EF-P, polymer, 189 residues, 21303.1 Da.

Natural source:

Natural source:   Common Name: Pseudomonas putida   Taxonomy ID: 303   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas putida

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EF-P: MKTGKELKPGTVLRIDNDPW LVQKAEFTKSGRNSAIMKTK LKNLLTGYKTETVYGADDKL DDVILDRKEATLSFINGDEY TFMDTTDYTMYELNAEDIEA VLPYIEEGMEDVCEAVFFEG RLVSVELPTTISRKVVYTEN AARGDTSGKVMKPAKLANGT EISVADFIQIDEWIDIDTRD NSFKGRSKK

Data sets:
Data typeCount
13C chemical shifts516
15N chemical shifts178
1H chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Elongation factor P1

Entities:

Entity 1, Elongation factor P 189 residues - 21303.1 Da.

1   METLYSTHRGLYLYSGLULEULYSPROGLY
2   THRVALLEUARGILEASPASNASPPROTRP
3   LEUVALGLNLYSALAGLUPHETHRLYSSER
4   GLYARGASNSERALAILEMETLYSTHRLYS
5   LEULYSASNLEULEUTHRGLYTYRLYSTHR
6   GLUTHRVALTYRGLYALAASPASPLYSLEU
7   ASPASPVALILELEUASPARGLYSGLUALA
8   THRLEUSERPHEILEASNGLYASPGLUTYR
9   THRPHEMETASPTHRTHRASPTYRTHRMET
10   TYRGLULEUASNALAGLUASPILEGLUALA
11   VALLEUPROTYRILEGLUGLUGLYMETGLU
12   ASPVALCYSGLUALAVALPHEPHEGLUGLY
13   ARGLEUVALSERVALGLULEUPROTHRTHR
14   ILESERARGLYSVALVALTYRTHRGLUASN
15   ALAALAARGGLYASPTHRSERGLYLYSVAL
16   METLYSPROALALYSLEUALAASNGLYTHR
17   GLUILESERVALALAASPPHEILEGLNILE
18   ASPGLUTRPILEASPILEASPTHRARGASP
19   ASNSERPHELYSGLYARGSERLYSLYS

Related Database Links:

UNP A0A059UQW3
AlphaFold A0A1L5PKK0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks