BMRB Entry 27148

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27148

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Title:
Backbone and side chain chemical shift assignments of wild-type PACT-D3.
Deposition date:
2017-06-19
Original release date:
2019-04-15
Authors:
Heyam, Alex
Citation:

Citation: Heyam, Alex; Coupland, Claire; Degut, Clement; Haley, Ruth; Baxter, Nicola; Jakob, Leonhard; Aguiar, Pedro; Meister, Gunter; Williamson, Michael; Lagos, Dimitris; Plevin, Michael. "Conserved asymmetry underpins homodimerization of Dicer-associated double-stranded RNA-binding proteins."  Nucleic Acids Res. 45, 12577-12584 (2017).
PubMed: 29045748

Assembly members:

Assembly members:
PACT-D3_wt, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETFPP_2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PACT-D3_wt: GPAMTDYIQLLSEIAKEQGF NITYLDIDELSANGQYQCLA ELSTSPITVCHGSGISCGNA QSDAAHNALQYLKIIAERK

Data sets:
Data typeCount
13C chemical shifts584
15N chemical shifts155
1H chemical shifts893

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PACT-D3 state_A1
2PACT-D3 state_B1

Entities:

Entity 1, PACT-D3 state_A 79 residues - Formula weight is not available

The first four residues (GPAM) are non-native, and remain after cleavage of an affinity tag. This is followed by PACT residues 239-313.

1   GLYPROALAMETTHRASPTYRILEGLNLEU
2   LEUSERGLUILEALALYSGLUGLNGLYPHE
3   ASNILETHRTYRLEUASPILEASPGLULEU
4   SERALAASNGLYGLNTYRGLNCYSLEUALA
5   GLULEUSERTHRSERPROILETHRVALCYS
6   HISGLYSERGLYILESERCYSGLYASNALA
7   GLNSERASPALAALAHISASNALALEUGLN
8   TYRLEULYSILEILEALAGLUARGLYS

Related Database Links:

UNP O75569
AlphaFold Q8NDK4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks