BMRB Entry 27175

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27175

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Title:
1H, 15N, 13C backbone resonance assignments of the D10N variant of beta-phosphoglucomutase in a MgII-free complex with beta-glucose 1,6-bisphosphate
Deposition date:
2017-07-13
Original release date:
2018-08-22
Authors:
Baxter, Nicola
Citation:

Citation: Johnson, Luke; Robertson, Angus; Baxter, Nicola; Trevitt, Clare; Bisson, Claudine; Jin, Yi; Hounslow, Andrea; Cliff, Matthew; Blackburn, Michael; Bowler, Matthew; Waltho, Jonathan. "van der Waals Contact between Nucleophile and Transferring Phosphorus Is Insufficient To Achieve Enzyme Transition-State Architecture"  ACS Catal. 8, 8140-8153 (2018).

Assembly members:

Assembly members:
beta-phosphoglucomutase, polymer, 221 residues, 24209.65 Da.
1,6-di-O-phosphono-beta-D-glucopyranose, non-polymer, 340.116 Da.

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta-phosphoglucomutase: MFKAVLFDLNGVITDTAEYH FRAWKALAEEIGINGVDRQF NEQLKGVSREDSLQKILDLA DKKVSAEEFKELAKRKNDNY VKMIQDVSPADVYPGILQLL KDLRSNKIKIALASASKNGP FLLERMNLTGYFDAIADPAE VAASKPAPDIFIAAAHAVGV APSESIGLEDSQAGIQAIKD SGALPIGVGRPEDLGDDIVI VPDTSHYTLEFLKEVWLQKQ K

Data sets:
Data typeCount
13C chemical shifts638
15N chemical shifts210
1H chemical shifts210
31P chemical shifts2

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1bPGM-D10N1
2bG16P2

Entities:

Entity 1, bPGM-D10N 221 residues - 24209.65 Da.

1   METPHELYSALAVALLEUPHEASPLEUASN
2   GLYVALILETHRASPTHRALAGLUTYRHIS
3   PHEARGALATRPLYSALALEUALAGLUGLU
4   ILEGLYILEASNGLYVALASPARGGLNPHE
5   ASNGLUGLNLEULYSGLYVALSERARGGLU
6   ASPSERLEUGLNLYSILELEUASPLEUALA
7   ASPLYSLYSVALSERALAGLUGLUPHELYS
8   GLULEUALALYSARGLYSASNASPASNTYR
9   VALLYSMETILEGLNASPVALSERPROALA
10   ASPVALTYRPROGLYILELEUGLNLEULEU
11   LYSASPLEUARGSERASNLYSILELYSILE
12   ALALEUALASERALASERLYSASNGLYPRO
13   PHELEULEUGLUARGMETASNLEUTHRGLY
14   TYRPHEASPALAILEALAASPPROALAGLU
15   VALALAALASERLYSPROALAPROASPILE
16   PHEILEALAALAALAHISALAVALGLYVAL
17   ALAPROSERGLUSERILEGLYLEUGLUASP
18   SERGLNALAGLYILEGLNALAILELYSASP
19   SERGLYALALEUPROILEGLYVALGLYARG
20   PROGLUASPLEUGLYASPASPILEVALILE
21   VALPROASPTHRSERHISTYRTHRLEUGLU
22   PHELEULYSGLUVALTRPLEUGLNLYSGLN
23   LYS

Entity 2, bG16P - C6 H14 O12 P2 - 340.116 Da.

1   B16

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