BMRB Entry 27187

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27187

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Drosophila melanogaster H2B
Deposition date:
2017-07-21
Original release date:
2018-06-21
Authors:
van Ingen, Hugo; Corbeski, Ivan
Citation:

Citation: Corbeski, Ivan; Dolinar, Klemen; Wienk, Hans; Boelens, Rolf; van Ingen, Hugo. "DNA repair factor APLF acts as a H2A-H2B histone chaperone through binding its DNA interaction surface"  Nucleic Acids Res. 46, 7138-7152 (2018).
PubMed: 29905837

Assembly members:

Assembly members:
H2B_in_the_H2A-H2B_complex, polymer, 123 residues, Formula weight is not available
H2A, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
H2B_in_the_H2A-H2B_complex: IPPKTSGKAAKKAGKAQKNI TKTDKKKKRKRKESYAIYIY KVLKQVHPDTGISSKAMSIM NSFVNDIFERIAAEASRLAH YNKRSTITSREIQTAVRLLL PGELAKHAVSEGTKAVTKYT SSK
H2A: SGRGKGGKVKGKAKSRSNRA GLQFPVGRIHRLLRKGNYAE RVGAGAPVYLAAVMEYLAAE VLELAGNAARDNKKTRIIPR HLQLAIRNDEELNKLLSGVT IAQGGVLPNIQAVLLPKKTE KKA

Data sets:
Data typeCount
13C chemical shifts342
15N chemical shifts112
1H chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1H2B1
2H2A2

Entities:

Entity 1, H2B 123 residues - Formula weight is not available

1   ILEPROPROLYSTHRSERGLYLYSALAALA
2   LYSLYSALAGLYLYSALAGLNLYSASNILE
3   THRLYSTHRASPLYSLYSLYSLYSARGLYS
4   ARGLYSGLUSERTYRALAILETYRILETYR
5   LYSVALLEULYSGLNVALHISPROASPTHR
6   GLYILESERSERLYSALAMETSERILEMET
7   ASNSERPHEVALASNASPILEPHEGLUARG
8   ILEALAALAGLUALASERARGLEUALAHIS
9   TYRASNLYSARGSERTHRILETHRSERARG
10   GLUILEGLNTHRALAVALARGLEULEULEU
11   PROGLYGLULEUALALYSHISALAVALSER
12   GLUGLYTHRLYSALAVALTHRLYSTYRTHR
13   SERSERLYS

Entity 2, H2A 123 residues - Formula weight is not available

1   SERGLYARGGLYLYSGLYGLYLYSVALLYS
2   GLYLYSALALYSSERARGSERASNARGALA
3   GLYLEUGLNPHEPROVALGLYARGILEHIS
4   ARGLEULEUARGLYSGLYASNTYRALAGLU
5   ARGVALGLYALAGLYALAPROVALTYRLEU
6   ALAALAVALMETGLUTYRLEUALAALAGLU
7   VALLEUGLULEUALAGLYASNALAALAARG
8   ASPASNLYSLYSTHRARGILEILEPROARG
9   HISLEUGLNLEUALAILEARGASNASPGLU
10   GLULEUASNLYSLEULEUSERGLYVALTHR
11   ILEALAGLNGLYGLYVALLEUPROASNILE
12   GLNALAVALLEULEUPROLYSLYSTHRGLU
13   LYSLYSALA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks