BMRB Entry 27313

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PDB ID: 6bqs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR27313
MolProbity Validation Chart

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Title:
HusA from porphyromonas gingivalis
Deposition date:
2017-11-14
Original release date:
2018-10-02
Authors:
Gell, David; Kwan, Ann
Citation:

Citation: Gao, Jin-Long; Kwan, Ann; Yammine, Anthony; Zhou, Xiaoyan; Trewhella, Jill; Hugrass, Barbara; Collins, Daniel; Horne, James; Ye, Ping; Harty, Derek; Nguyen, Ky-Anh; Gell, David; Hunter, Neil. "Structural properties of a haemophore facilitate targeted elimination of the pathogen Porphyromonas gingivalis"  Nat. Commun. 9, 4097-4097 (2018).
PubMed: 30291238

Assembly members:

Assembly members:
HusA, polymer, 201 residues, 21726.6 Da.

Natural source:

Natural source:   Common Name: Porphyromonas gingivalis   Taxonomy ID: 837   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Porphyromonas gingivalis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HusA: MGQGTAYAEVMNRKVAALDS VPPTEYATLAADFSRIAAVE GSDWMAAYYTAYCRIIPAFG NPSEADRLCEEAESMLSKAE SLGGDLSEIACLRSMAASAR LLVNPQERWQTYGAESSRQL AVALEANPANPRAYFLQAQS LLYTPAQFGGGKDKALPFAE KSVSCYAAATVSPAYAPHWG EQQARQLLMLCKAETQELVP R

Data sets:
Data typeCount
13C chemical shifts622
15N chemical shifts201
1H chemical shifts1333

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HusA trans-Pro2001
2HusA cis-Pro2001

Entities:

Entity 1, HusA trans-Pro200 201 residues - 21726.6 Da.

Residues 1, 2 are from the expression vector. Residues 3 to 196 correspond to UniProt: Q7MSY3, residues 24-217 Porphyromonas gingivalis gene PG_2227 Residues 197-201 are derived from the expression vector

1   METGLYGLNGLYTHRALATYRALAGLUVAL
2   METASNARGLYSVALALAALALEUASPSER
3   VALPROPROTHRGLUTYRALATHRLEUALA
4   ALAASPPHESERARGILEALAALAVALGLU
5   GLYSERASPTRPMETALAALATYRTYRTHR
6   ALATYRCYSARGILEILEPROALAPHEGLY
7   ASNPROSERGLUALAASPARGLEUCYSGLU
8   GLUALAGLUSERMETLEUSERLYSALAGLU
9   SERLEUGLYGLYASPLEUSERGLUILEALA
10   CYSLEUARGSERMETALAALASERALAARG
11   LEULEUVALASNPROGLNGLUARGTRPGLN
12   THRTYRGLYALAGLUSERSERARGGLNLEU
13   ALAVALALALEUGLUALAASNPROALAASN
14   PROARGALATYRPHELEUGLNALAGLNSER
15   LEULEUTYRTHRPROALAGLNPHEGLYGLY
16   GLYLYSASPLYSALALEUPROPHEALAGLU
17   LYSSERVALSERCYSTYRALAALAALATHR
18   VALSERPROALATYRALAPROHISTRPGLY
19   GLUGLNGLNALAARGGLNLEULEUMETLEU
20   CYSLYSALAGLUTHRGLNGLULEUVALPRO
21   ARG

Related Database Links:

NCBI PG_RS09910
GB AAQ67167.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks