BMRB Entry 27399

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27399

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Title:
A low-complexity region in the YTH domain protein Mmi1 enhances RNA binding
Deposition date:
2018-02-08
Original release date:
2018-04-24
Authors:
Stowell, James; Wagstaff, Jane; Hill, Chris; Yu, Minmin; McLaughlin, Stephen; Freund, Stefan; Passmore, Lori
Citation:

Citation: Stowell, James; Wagstaff, Jane; Hill, Chris; Yu, Minmin; McLaughlin, Stephen; Freund, Stefan; Passmore, Lori. "A low-complexity region in the YTH domain protein Mmi1 enhances RNA binding."  J. Biol. Chem. 293, 9210-9222 (2018).
PubMed: 29695507

Assembly members:

Assembly members:
USR+YTH_domain, polymer, 207 residues, Formula weight is not available
19mer_DSR_RNA, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
USR+YTH_domain: GAYPEKSSYLLSNSSNDSAS RKEKPKARASTPPPLNFSRA SEHRNEKGERISMINPRVVL DENGISHRSRYFIMLCDNET AIAHAKKTSIWAVKKDSSKR ISDAYKKASVYFIFVAQQTY NALGYAQVVSDLNSTELPFW SDSSHAGGVRIKWIKTCNLF SAEISEIVSHMDHGSEARDG MEMMYDEGSRLCTLINYAIM KRIGRDR
19mer_DSR_RNA: CUCUUUAAACCUAAAAAGC

Data sets:
Data typeCount
13C chemical shifts440
15N chemical shifts138
1H chemical shifts138

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