BMRB Entry 27483

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27483

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Title:
Backbone assignment of ICP27 1-138
Deposition date:
2018-05-15
Original release date:
2018-09-24
Authors:
Tunnicliffe, Richard; Tian, Xiaochen; Storer, Joanna; Sandri-Goldin, Rozanne; Golovanov, Alexander
Citation:

Citation: Tunnicliffe, Richard; Tian, Xiaochen; Storer, Joanna; Sandri-Goldin, Rozanne; Golovanov, Alexander. "Overlapping motifs on the herpes viral proteins ICP27 and ORF57 mediate interactions with the mRNA export adaptors ALYREF and UIF"  Sci. Rep. 8, 15005-15005 (2018).

Assembly members:

Assembly members:
ICP27, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Herpes simplex virus 1   Taxonomy ID: 10298   Superkingdom: Viruses   Kingdom: not available   Genus/species: Simplexvirus Herpes simplex virus 1

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ICP27: MATDIDMLIDLGLDLSDSDL DEDPPEPAESRRDDLESDSN GECSSSDEDMEDPHGEDGPE PILDAARPAVRPSRPEDPGV PSTQTPRPTERQGPNDPQPA PHSVWSRLGARRPSCSPERH GGKVARLQPPPTKAQPARKL LEVLFQGPLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts391
15N chemical shifts140
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ICP27 1-1381

Entities:

Entity 1, ICP27 1-138 156 residues - Formula weight is not available

Residues 139-156 are not wild-type and introduced during cloning

1   METALATHRASPILEASPMETLEUILEASP
2   LEUGLYLEUASPLEUSERASPSERASPLEU
3   ASPGLUASPPROPROGLUPROALAGLUSER
4   ARGARGASPASPLEUGLUSERASPSERASN
5   GLYGLUCYSSERSERSERASPGLUASPMET
6   GLUASPPROHISGLYGLUASPGLYPROGLU
7   PROILELEUASPALAALAARGPROALAVAL
8   ARGPROSERARGPROGLUASPPROGLYVAL
9   PROSERTHRGLNTHRPROARGPROTHRGLU
10   ARGGLNGLYPROASNASPPROGLNPROALA
11   PROHISSERVALTRPSERARGLEUGLYALA
12   ARGARGPROSERCYSSERPROGLUARGHIS
13   GLYGLYLYSVALALAARGLEUGLNPROPRO
14   PROTHRLYSALAGLNPROALAARGLYSLEU
15   LEUGLUVALLEUPHEGLNGLYPROLEUGLU
16   HISHISHISHISHISHIS

Related Database Links:

UNP Q9J0X9
AlphaFold Q9J0X9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks