BMRB Entry 27548

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27548

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Title:
Ressonance assignments for the human Smad5 MH1 domain
Deposition date:
2018-07-17
Original release date:
2018-11-15
Authors:
Macias, Maria; Martin-Malpartida, Pau; Ruiz, Lidia; Aragon, Eric; Gomes, Tiago
Citation:

Citation: Guca, Ewelina; Sunol, David; Ruiz, Lidia; Konkol, Agnieszka; Cordero, Jorge; Torner, Carles; Aragon, Eric; Martin-Malpartida, Pau; Riera, Antoni; Macias, Maria. "TGIF1 homeodomain interacts with Smad MH1 domain and represses TGF-beta signaling."  Nucleic Acids Res. 46, 9220-9235 (2018).
PubMed: 30060237

Assembly members:

Assembly members:
SMAD5-MH1, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SMAD5-MH1: GPSFTSPAVKRLLGWKQGDE EEKWAEKAVDALVKKLKKKK GAMEELEKALSSPGQPSKCV TIPRSLDGRLQVSHRKGLPH VIYCRVWRWPDLQSHHELKP LDICEFPFGSKQKEVCINPY HYKRVESPVLPPVLVPR

Data sets:
Data typeCount
13C chemical shifts196
15N chemical shifts84
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SMAD5-MH11

Entities:

Entity 1, SMAD5-MH1 137 residues - Formula weight is not available

1   GLYPROSERPHETHRSERPROALAVALLYS
2   ARGLEULEUGLYTRPLYSGLNGLYASPGLU
3   GLUGLULYSTRPALAGLULYSALAVALASP
4   ALALEUVALLYSLYSLEULYSLYSLYSLYS
5   GLYALAMETGLUGLULEUGLULYSALALEU
6   SERSERPROGLYGLNPROSERLYSCYSVAL
7   THRILEPROARGSERLEUASPGLYARGLEU
8   GLNVALSERHISARGLYSGLYLEUPROHIS
9   VALILETYRCYSARGVALTRPARGTRPPRO
10   ASPLEUGLNSERHISHISGLULEULYSPRO
11   LEUASPILECYSGLUPHEPROPHEGLYSER
12   LYSGLNLYSGLUVALCYSILEASNPROTYR
13   HISTYRLYSARGVALGLUSERPROVALLEU
14   PROPROVALLEUVALPROARG

Related Database Links:

UNP Q99717
AlphaFold Q9UQA1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks