BMRB Entry 27573

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27573

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Title:
Backbone and side chain chemical shifts assignments of uniformly labeled 13C, 15N SUMO1-F64L
Deposition date:
2018-08-08
Original release date:
2019-03-01
Authors:
Das, Ranabir; Chatterjee, Kiran Sankar
Citation:

Citation: Chatterjee, Kiran Sankar; Tripathi, Vasvi; Das, Ranabir. "A conserved and buried edge-to-face aromatic interaction in small ubiquitin-like modifier (SUMO) has a role in SUMO stability and function"  J. Biol. Chem. 294, 6772-6784 (2019).
PubMed: 30824543

Assembly members:

Assembly members:
SUMO1_F64L, polymer, 109 residues, 12497 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE-80L

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SUMO1_F64L: MRGSHHHHHHGSMSDQEAKP STEDLGDKKEGEYIKLKVIG QDSSEIHFKVKMTTHLKKLK ESYCQRQGVPMNSLRLLFEG QRIADNHTPKELGMEEEDVI EVYQEQTGG

Data sets:
Data typeCount
13C chemical shifts197
15N chemical shifts86
1H chemical shifts472

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SUMO1 F64L1

Entities:

Entity 1, SUMO1 F64L 109 residues - 12497 Da.

residue 1 to 12 is His affinity tag

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERMETSERASPGLNGLUALALYSPRO
3   SERTHRGLUASPLEUGLYASPLYSLYSGLU
4   GLYGLUTYRILELYSLEULYSVALILEGLY
5   GLNASPSERSERGLUILEHISPHELYSVAL
6   LYSMETTHRTHRHISLEULYSLYSLEULYS
7   GLUSERTYRCYSGLNARGGLNGLYVALPRO
8   METASNSERLEUARGLEULEUPHEGLUGLY
9   GLNARGILEALAASPASNHISTHRPROLYS
10   GLULEUGLYMETGLUGLUGLUASPVALILE
11   GLUVALTYRGLNGLUGLNTHRGLYGLY

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks