BMRB Entry 27599

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27599

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Title:
Backbone chemical shifts for super-stable p53 DNA-Binding Domain C5xS
Deposition date:
2018-09-05
Original release date:
2018-11-07
Authors:
Krois, Alexander; Dyson, Jane; Wright, Peter
Citation:

Citation: Krois, Alexander; Dyson, Jane; Wright, Peter. "Long-range regulation of p53 DNA binding by its intrinsically disordered N-terminal transactivation domain"  Proc. Natl. Acad. Sci. U.S.A. 115, E11302-E11310 (2018).
PubMed: 30420502

Assembly members:

Assembly members:
p53(DBD)_super-stable_C5xS, polymer, 228 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p53(DBD)_super-stable_C5xS: GSHAPSWPLSSSVPSQKTYQ GSYGFRLGFLHSGTAKSVTS TYSPALNKLFCQLAKTCPVQ LWVDSTPPPGTRVRAMAIYK QSQHMTEVVRRCPHHERSSD SDGLAPPQHLIRVEGNLRAE YLDDRNTFRHSVVVPYEPPE VGSDSTTIHYNYMCYSSCMG GMNRRPILTIITLEDSSGNL LGRDSFEVRVSASPGRDRRT EEENLRKKGEPHHELPPGST KRALPNNT

Data sets:
Data typeCount
13C chemical shifts375
15N chemical shifts176
1H chemical shifts176

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1p53(DBD)1

Entities:

Entity 1, p53(DBD) 228 residues - Formula weight is not available

Residues 85, 86, and 87 are from expression/cloning and are not a part of the native sequence.

1   GLYSERHISALAPROSERTRPPROLEUSER
2   SERSERVALPROSERGLNLYSTHRTYRGLN
3   GLYSERTYRGLYPHEARGLEUGLYPHELEU
4   HISSERGLYTHRALALYSSERVALTHRSER
5   THRTYRSERPROALALEUASNLYSLEUPHE
6   CYSGLNLEUALALYSTHRCYSPROVALGLN
7   LEUTRPVALASPSERTHRPROPROPROGLY
8   THRARGVALARGALAMETALAILETYRLYS
9   GLNSERGLNHISMETTHRGLUVALVALARG
10   ARGCYSPROHISHISGLUARGSERSERASP
11   SERASPGLYLEUALAPROPROGLNHISLEU
12   ILEARGVALGLUGLYASNLEUARGALAGLU
13   TYRLEUASPASPARGASNTHRPHEARGHIS
14   SERVALVALVALPROTYRGLUPROPROGLU
15   VALGLYSERASPSERTHRTHRILEHISTYR
16   ASNTYRMETCYSTYRSERSERCYSMETGLY
17   GLYMETASNARGARGPROILELEUTHRILE
18   ILETHRLEUGLUASPSERSERGLYASNLEU
19   LEUGLYARGASPSERPHEGLUVALARGVAL
20   SERALASERPROGLYARGASPARGARGTHR
21   GLUGLUGLUASNLEUARGLYSLYSGLYGLU
22   PROHISHISGLULEUPROPROGLYSERTHR
23   LYSARGALALEUPROASNASNTHR

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks