BMRB Entry 27640

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27640

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Title:
Chemical Shifts of KCNE1TVG in LMPG micelles
Deposition date:
2018-10-08
Original release date:
2018-10-12
Authors:
Law, Cheryl; Sanders, Charles
Citation:

Citation: Law, Cheryl; Sanders, Charles. "NMR resonance assignments and secondary structure of a mutant form of the human KCNE1 channel accessory protein that exhibits KCNE3-like function."  Biomol. NMR Assignments 13, 143-147 (2019).
PubMed: 30603955

Assembly members:

Assembly members:
KCNE1TVG, polymer, 136 residues, 15434.45 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KCNE1TVG: HHHHHHGMILSNTTAVTPFL TKLWQETVQQGGNMSGLARR SPRSGDGKLEALYVLMVLGF FGFTVGGIMLSYIRSKKLEH SNDPFNVYIESDAWQEKDKA YVQARVLESYRSSYVVENHL AIEQPNTHLPETKPSP

Data sets:
Data typeCount
13C chemical shifts300
15N chemical shifts111
1H chemical shifts118

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LMPG detergent1

Entities:

Entity 1, LMPG detergent 136 residues - 15434.45 Da.

Residues 1-7 represent a non native affinity tag

1   HISHISHISHISHISHISGLYMETILELEU
2   SERASNTHRTHRALAVALTHRPROPHELEU
3   THRLYSLEUTRPGLNGLUTHRVALGLNGLN
4   GLYGLYASNMETSERGLYLEUALAARGARG
5   SERPROARGSERGLYASPGLYLYSLEUGLU
6   ALALEUTYRVALLEUMETVALLEUGLYPHE
7   PHEGLYPHETHRVALGLYGLYILEMETLEU
8   SERTYRILEARGSERLYSLYSLEUGLUHIS
9   SERASNASPPROPHEASNVALTYRILEGLU
10   SERASPALATRPGLNGLULYSASPLYSALA
11   TYRVALGLNALAARGVALLEUGLUSERTYR
12   ARGSERSERTYRVALVALGLUASNHISLEU
13   ALAILEGLUGLNPROASNTHRHISLEUPRO
14   GLUTHRLYSPROSERPRO

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks