BMRB Entry 27705

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27705

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Guanylate Cyclase Activating Protein-5 (GCAP5) in Zebrafish Photoreceptors
Deposition date:
2018-11-26
Original release date:
2019-01-08
Authors:
Cudia, Diana; Ames, James
Citation:

Citation: Peshenko, Igor; Yu, Qinhong; Lim, Sunghyuk; Cudia, Diana; Dizhoor, Alexander; Ames, James. "Retinal degeneration 3 (RD3) protein, a retinal guanylyl cyclase regulator, forms a monomeric and elongated four helix bundle"  J. Biol. Chem. 94, 2318-2328 (2019).
PubMed: 30559291

Assembly members:

Assembly members:
GCAP5, polymer, 198 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GCAP5: MGDSSSMSATELSACKCHQW YRKFMTECPSGQLTFYEFKK FFGLKNLSEKSNAYVNTMFK TFDIDDDGCIDFMEYVAALS LVLKGGVQQKLRWYFKLFDM DGSGCIDKDELLLIFKAVQA INGAEPEISAEDLADIVFNK IDVNGDGELSLEEFMEGISA DEKISEMLTQSLDLTRIVSN IYNDSYIEQEAEIIEDQA

Data sets:
Data typeCount
13C chemical shifts579
15N chemical shifts160
1H chemical shifts812

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GCAP51

Entities:

Entity 1, GCAP5 198 residues - Formula weight is not available

1   METGLYASPSERSERSERMETSERALATHR
2   GLULEUSERALACYSLYSCYSHISGLNTRP
3   TYRARGLYSPHEMETTHRGLUCYSPROSER
4   GLYGLNLEUTHRPHETYRGLUPHELYSLYS
5   PHEPHEGLYLEULYSASNLEUSERGLULYS
6   SERASNALATYRVALASNTHRMETPHELYS
7   THRPHEASPILEASPASPASPGLYCYSILE
8   ASPPHEMETGLUTYRVALALAALALEUSER
9   LEUVALLEULYSGLYGLYVALGLNGLNLYS
10   LEUARGTRPTYRPHELYSLEUPHEASPMET
11   ASPGLYSERGLYCYSILEASPLYSASPGLU
12   LEULEULEUILEPHELYSALAVALGLNALA
13   ILEASNGLYALAGLUPROGLUILESERALA
14   GLUASPLEUALAASPILEVALPHEASNLYS
15   ILEASPVALASNGLYASPGLYGLULEUSER
16   LEUGLUGLUPHEMETGLUGLYILESERALA
17   ASPGLULYSILESERGLUMETLEUTHRGLN
18   SERLEUASPLEUTHRARGILEVALSERASN
19   ILETYRASNASPSERTYRILEGLUGLNGLU
20   ALAGLUILEILEGLUASPGLNALA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks