BMRB Entry 27781
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PDB ID: 6vk2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27781
MolProbity Validation Chart
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NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Sanches, Karoline; Caruso, Icaro; Almeida, Fabio; Melo, Fernando. "NMR assignment of free 1H, 15N and 13C-Grb2-SH2 domain" Biomol. NMR Assignments 13, 295-298 (2019).
PubMed: 31028611
Assembly members:
SH2-Grb2, polymer, 106 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 33
Entity Sequences (FASTA):
SH2-Grb2: MGSSHHHHHHMKPHPWFFGK
IPRAKAEEMLSKQRHDGAFL
IRESESAPGDFSLSVKFGND
VQHFKVLRDGAGKYFLWVVK
FNSLNELVDYHRSTSVSRNQ
QIFLRD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 414 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 633 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH2 domain | 1 |
Entities:
Entity 1, SH2 domain 106 residues - Formula weight is not available
Residues 1-10 represent a non-native affinity tag. In the manuscript residue 1 start at MKPH....
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | MET | LYS | PRO | HIS | PRO | TRP | PHE | PHE | GLY | LYS | ||||
| 3 | ILE | PRO | ARG | ALA | LYS | ALA | GLU | GLU | MET | LEU | ||||
| 4 | SER | LYS | GLN | ARG | HIS | ASP | GLY | ALA | PHE | LEU | ||||
| 5 | ILE | ARG | GLU | SER | GLU | SER | ALA | PRO | GLY | ASP | ||||
| 6 | PHE | SER | LEU | SER | VAL | LYS | PHE | GLY | ASN | ASP | ||||
| 7 | VAL | GLN | HIS | PHE | LYS | VAL | LEU | ARG | ASP | GLY | ||||
| 8 | ALA | GLY | LYS | TYR | PHE | LEU | TRP | VAL | VAL | LYS | ||||
| 9 | PHE | ASN | SER | LEU | ASN | GLU | LEU | VAL | ASP | TYR | ||||
| 10 | HIS | ARG | SER | THR | SER | VAL | SER | ARG | ASN | GLN | ||||
| 11 | GLN | ILE | PHE | LEU | ARG | ASP |
Download HSQC peak lists in one of the following formats:
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