BMRB Entry 30101

Assembly:

Entities:

Entity 1, entity_1 217 residues - 25348.918 Da.

Samples:

sample_1: melanoregulin, [U-99% 15N], 0.150 mM; H2O 90%; D2O 10%; L-Arginine 5 mM; L-Glutamine 5 mM

sample_2: melanoregulin, [U-99% 13C; U-99% 15N], 0.150 mM; H2O 90%; D2O 10%; L-Arginine 5 mM; L-Glutamine 5 mM

sample_3: melanoregulin, U-15;U-13C;U-2H, 0.150 mM; H2O 90%; D2O 10%; L-Arginine 5 mM; L-Glutamine 5 mM

sample_4: melanoregulin, 15N/13C/2H/ILV-CH3, 0.150 mM; D2O 100%; L-Arginine 5 mM; L-Glutamine 5 mM

sample_5: melanoregulin, [U-99% 15N], 0.150 mM; H2O 90%; D2O 10%; L-Arginine 5 mM; L-Glutamine 5 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.3; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 10 mM; pH: 6.3; pressure: 1 atm; temperature: 303 K

Experiments:

Software:

CARA, Keller and Wuthrich - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PIPP, Garrett - data analysis

TOPSPIN, Bruker Biospin - collection

XPLOR, G. Marius Clore , Guillermo Bermejo, , John Kuszewski, Charles D. Schwieters, and Nico Tjandra - structure calculation

NMR spectrometers:

• Bruker Avance 900 MHz
• Bruker Avance 800 MHz
• Bruker Avance 600 MHz