BMRB Entry 30457

Title:
HSP40 co-chaperone Sis1 J-domain
Deposition date:
2018-04-23
Original release date:
2019-04-24
Authors:
Pinheiro, G.; Amorim, G.; Iqbal, A.; Ramos, C.; Almeida, F.
Citation:

Citation: Pinheiro, Glaucia; Amorim, Gisele; Iqbal, Anwar; Almeida, Fabio; Ramos, C. "Solution NMR investigation on the structure and function of the isolated J-domain from Sis1: Evidence of transient inter-domain interactions in the full-length protein"  Arch. Biochem. Biophys. 669, 71-79 (2019).
PubMed: 31141701

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, 9091.122 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pEt32a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts323
15N chemical shifts73
1H chemical shifts467

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 81 residues - 9091.122 Da.

1   SERVALLYSGLUTHRLYSLEUTYRASPLEU
2   LEUGLYVALSERPROSERALAASNGLUGLN
3   GLULEULYSLYSGLYTYRARGLYSALAALA
4   LEULYSTYRHISPROASPLYSPROTHRGLY
5   ASPTHRGLULYSPHELYSGLUILESERGLU
6   ALAPHEGLUILELEUASNASPPROGLNLYS
7   ARGGLUILETYRASPGLNTYRGLYLEUGLU
8   ALAALAARGSERGLYGLYPROSERPHEGLY
9   PRO

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