BMRB Entry 4131

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4131

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Title:
1H, 15N, 13C chemical shifts of recombinant rat ferrocytochrome b5, B conformation
Deposition date:
1998-04-07
Original release date:
1998-08-12
Authors:
Dangi, Bindi; Sarma, Siddhartha; Yan, Chunhua; Banville, Debra; Guiles, Ronald
Citation:

Citation: Dangi, Bindi; Sarma, Siddhartha; Yan, Chunhua; Banville, Debra; Guiles, Ronald. "The Origin of Difference in the Physical Properties of the Equilibrium Forms of Cytochrome b5 Revealed Through High Resolution NMR Structures and Backbone Dynamic Analyses"  Biochemistry 37, 8289-8302 (1998).

Assembly members:

Assembly members:
cytochrome b5, polymer, 94 residues, Formula weight is not available
HEME C, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: rat   Taxonomy ID: 10117   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus rattus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cytochrome b5: DKDVKYYTLEEIQKHKDSKS TWVILHHKVYDLTKFLEEHP GGEEVLREQAGGDATENFED VGHSTDARELSKTYIIGELH PDDRSKIAKPSETL

Data sets:
Data typeCount
1H chemical shifts514
15N chemical shifts90
13C chemical shifts297

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cytb5_B1
2heme2

Entities:

Entity 1, cytb5_B 94 residues - Formula weight is not available

1   ASPLYSASPVALLYSTYRTYRTHRLEUGLU
2   GLUILEGLNLYSHISLYSASPSERLYSSER
3   THRTRPVALILELEUHISHISLYSVALTYR
4   ASPLEUTHRLYSPHELEUGLUGLUHISPRO
5   GLYGLYGLUGLUVALLEUARGGLUGLNALA
6   GLYGLYASPALATHRGLUASNPHEGLUASP
7   VALGLYHISSERTHRASPALAARGGLULEU
8   SERLYSTHRTYRILEILEGLYGLULEUHIS
9   PROASPASPARGSERLYSILEALALYSPRO
10   SERGLUTHRLEU

Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Related Database Links:

BMRB 4130
PDB 1AQA 1AW3 1AXX 1B5A 1B5B 1BFX 1BLV 1I87 1I8C 1IB7 1IET 1IEU 1JEX 1MNY 2AXX
DBJ BAA02492 BAB22093 BAB28714 BAE20982
GB AAA67175 AAA72420 AAA72421 AAA72557 AAA99718
REF NP_001268328 NP_071581 NP_080073 XP_003512427 XP_005356388
SP P00173 P56395
AlphaFold P00173 P56395

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks