BMRB Entry 4254

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PDB ID: 1l3g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4254
MolProbity Validation Chart

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Title:
1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Sassharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Free DNA
Deposition date:
1998-10-27
Original release date:
1999-10-26
Authors:
McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew
Citation:

Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Saccharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Fee DNA"  J. Biomol. NMR 13, 397-398 (1999).

Assembly members:

Assembly members:
MLU1-BOX binding protein, polymer, 135 residues, 15436.7 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET22B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MLU1-BOX binding protein: SNQIYSARYSGVDVYEFIHS TGSIMKRKKDDWVNATHILK AANFAKAKRTRILEKEVLKE THEKVQGGFGKYQGTWVPLN IAKQLAEKFSVYDQLKPLFD FTQTDGSASPPPAPKHHHAS KVDKLAAALEHHHHH

Data sets:
Data typeCount
13C chemical shifts242
15N chemical shifts126
1H chemical shifts488

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Mbp11

Entities:

Entity 1, Mbp1 135 residues - 15436.7 Da.

1   SERASNGLNILETYRSERALAARGTYRSER
2   GLYVALASPVALTYRGLUPHEILEHISSER
3   THRGLYSERILEMETLYSARGLYSLYSASP
4   ASPTRPVALASNALATHRHISILELEULYS
5   ALAALAASNPHEALALYSALALYSARGTHR
6   ARGILELEUGLULYSGLUVALLEULYSGLU
7   THRHISGLULYSVALGLNGLYGLYPHEGLY
8   LYSTYRGLNGLYTHRTRPVALPROLEUASN
9   ILEALALYSGLNLEUALAGLULYSPHESER
10   VALTYRASPGLNLEULYSPROLEUPHEASP
11   PHETHRGLNTHRASPGLYSERALASERPRO
12   PROPROALAPROLYSHISHISHISALASER
13   LYSVALASPLYSLEUALAALAALALEUGLU
14   HISHISHISHISHIS

Related Database Links:

BMRB 4256
PDB 1BM8 1L3G
DBJ GAA22190
EMBL CAA52271 CAA98618 CAY78452
GB AAS64332 AAS64333 AAS64335 AAS64336 AAS64338
REF NP_010227
SP P39678
TPG DAA11800
AlphaFold P39678

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