BMRB Entry 4445

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PDB ID: 1d8b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4445
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments and coupling constants for the HRDC domain from S. cerevisiae Sgs1 RecQ helicase
Deposition date:
1999-10-22
Original release date:
2000-07-18
Authors:
Liu, Zhihong; Macias, Maria; Bottomley, Matthew; Stier, Gunter; Linge, Jens; Nilges, Michael; Bork, Peer; Sattler, Michael
Citation:

Citation: Liu, Zhihong; Macias, Maria; Bottomley, Matthew; Stier, Gunter; Linge, Jens; Nilges, Michael; Bork, Peer; Sattler, Michael. "The 3D structure of the HRDC domain and implications for the Werner and Bloom syndrome proteins."  Structure 7, 1557-1566 (1999).

Assembly members:

Assembly members:
HRDC domain of Sgs1 RecQ helicase, polymer, 91 residues, 10681 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET9D

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HRDC domain of Sgs1 RecQ helicase: MKHHHHHHPMELNNLRMTYE RLRELSLNLGNRMVPPVGNF MPDSILKKMAAILPMNDSAF ATLGTVEDKYRRRFKYFKAT IADLSKKRSSE

Data sets:
Data typeCount
1H chemical shifts1087
13C chemical shifts289
15N chemical shifts158
coupling constants53

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HRDC1

Entities:

Entity 1, HRDC 91 residues - 10681 Da.

1   METLYSHISHISHISHISHISHISPROMET
2   GLULEUASNASNLEUARGMETTHRTYRGLU
3   ARGLEUARGGLULEUSERLEUASNLEUGLY
4   ASNARGMETVALPROPROVALGLYASNPHE
5   METPROASPSERILELEULYSLYSMETALA
6   ALAILELEUPROMETASNASPSERALAPHE
7   ALATHRLEUGLYTHRVALGLUASPLYSTYR
8   ARGARGARGPHELYSTYRPHELYSALATHR
9   ILEALAASPLEUSERLYSLYSARGSERSER
10   GLU

Related Database Links:

PDB 1D8B
DBJ GAA25638
EMBL CAA87811 CAY82020
GB AAA35167 AAB60289 AHY76643 AJP40883 AJS62054
REF NP_013915
SP P35187
TPG DAA10088
AlphaFold P35187

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