BMRB Entry 4448

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PDB ID: 1f3y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4448
MolProbity Validation Chart

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Title:
1H, 13C and 15N backbone assignment and secondary structure of the 19 kDa diadenosine 5',5'''-P1,P4 tetraphosphate hydrolase from Lupinus angustifolius L.
Deposition date:
1999-11-01
Original release date:
2000-05-09
Authors:
Swarbrick, James; Bashtannyk, Tanya; Maksel, Danuta; Gayler, Kenwyn; Pau, Richard; Gooley, Paul
Citation:

Citation: Swarbrick, James; Bashtannyk, Tanya; Maksel, Danuta; Gayler, Kenwyn; Pau, Richard; Gooley, Paul. "Letter to the Editor: 1H, 13C and 15N backbone assignment and secondary structure of the 19 kDa diadenosine 5', 5'''-P1, P4-tetraphosphate hydrolase from Lupinus angustifolius L"  J. Biomol. NMR 16, 269-270 (2000).

Assembly members:

Assembly members:
Ap4a hydrolase, polymer, 165 residues, 18815.2 Da.

Natural source:

Natural source:   Common Name: lupin   Taxonomy ID: 3871   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Lupinus angustifolius

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ap4a hydrolase: GPLGSMDSPPEGYRRNVGIC LMNNDKKIFAASRLDIPDAW QMPQGGIDEGEDPRNAAIRE LREETGVTSAEVIAEVPYWL TYDFPPKVREKLNIQWGSDW KGQAQKWFLFKFTGQDQEIN LLGDGSEKPEFGEWSWVTPE QLIDLTVEFKKPVYKEVLSV FAPHL

Data sets:
Data typeCount
1H chemical shifts1014
13C chemical shifts612
15N chemical shifts162

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ap4a hydrolase monomer1

Entities:

Entity 1, Ap4a hydrolase monomer 165 residues - 18815.2 Da.

1   GLYPROLEUGLYSERMETASPSERPROPRO
2   GLUGLYTYRARGARGASNVALGLYILECYS
3   LEUMETASNASNASPLYSLYSILEPHEALA
4   ALASERARGLEUASPILEPROASPALATRP
5   GLNMETPROGLNGLYGLYILEASPGLUGLY
6   GLUASPPROARGASNALAALAILEARGGLU
7   LEUARGGLUGLUTHRGLYVALTHRSERALA
8   GLUVALILEALAGLUVALPROTYRTRPLEU
9   THRTYRASPPHEPROPROLYSVALARGGLU
10   LYSLEUASNILEGLNTRPGLYSERASPTRP
11   LYSGLYGLNALAGLNLYSTRPPHELEUPHE
12   LYSPHETHRGLYGLNASPGLNGLUILEASN
13   LEULEUGLYASPGLYSERGLULYSPROGLU
14   PHEGLYGLUTRPSERTRPVALTHRPROGLU
15   GLNLEUILEASPLEUTHRVALGLUPHELYS
16   LYSPROVALTYRLYSGLUVALLEUSERVAL
17   PHEALAPROHISLEU

Related Database Links:

PDB 1F3Y 1JKN
GB AAC49902

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks