BMRB Entry 4802

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4802

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Title:
1H, 13C, and 15N sequential assignment of the triple labelled N-terminal domain of the Histone like Nucleoid Structuring protein (H-NS) from Salmonella typhimurium (first 64 residues of the protein)
Deposition date:
2000-08-09
Original release date:
2001-01-23
Authors:
Renzoni, Debora; Esposito, Diego; Pfuhl, Mark; Higgins, Christopher; Driscoll, Paul; Ladbury, John
Citation:

Citation: Renzoni, Debora; Esposito, Diego; Pfuhl, Mark; Higgins, Christopher; Driscoll, Paul; Ladbury, John. "Structural Insight to the Oligomerisation of the Bacterial Chromatin-Structuring Protein H-N"  .

Assembly members:

Assembly members:
Oligomerisation domain of the 'histone' like nucleoid structuring protein, polymer, 64 residues, 7958 Da.

Natural source:

Natural source:   Common Name: Salmonella typhimurium   Taxonomy ID: 602   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Salmonella typhimurium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Oligomerisation domain of the 'histone' like nucleoid structuring protein: SEALKILNNIRTLRAQARES TLETLEEMLEKLEVVVNERR EEESAAAAEVEERTRKLQQY REML

Data sets:
Data typeCount
1H chemical shifts123
13C chemical shifts168
15N chemical shifts62

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1H-NS(1-64) monomer 11
2H-NS(1-64) monomer 21
3H-NS(1-64) monomer 31

Entities:

Entity 1, H-NS(1-64) monomer 1 64 residues - 7958 Da.

1   SERGLUALALEULYSILELEUASNASNILE
2   ARGTHRLEUARGALAGLNALAARGGLUSER
3   THRLEUGLUTHRLEUGLUGLUMETLEUGLU
4   LYSLEUGLUVALVALVALASNGLUARGARG
5   GLUGLUGLUSERALAALAALAALAGLUVAL
6   GLUGLUARGTHRARGLYSLEUGLNGLNTYR
7   ARGGLUMETLEU

Related Database Links:

BMRB 18814 5390
PDB 1LR1 1NI8 3NR7
DBJ BAA36117 BAB35162 BAG76811 BAI25048 BAI30173
EMBL CAA30530 CAA31522 CAA32549 CAA40507 CAA42565
GB AAB61148 AAC74319 AAG56094 AAL20669 AAN42850
PIR AC0650
PRF 1607341A
REF NP_287482 NP_309766 NP_415753 NP_455749 NP_460710
SP P09120 P0A1S2 P0A1S3 P0ACF8 P0ACF9
AlphaFold P09120 P0A1S2 P0A1S3 P0ACF8 P0ACF9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks