BMRB Entry 5218

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5218

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Title:
Backbone 1H, 13C, 15N resonance assignments of the N-terminal 24 kDa fragment of the gyrase B subunit from E. coli
Deposition date:
2001-11-23
Original release date:
2002-08-22
Authors:
Bellanda, Massimo; Peggion, Evaristo; Otting, Gottfried; Weigelt, Johan; Perdona, Elisabetta; Domenici, Enrico; Marchioro, Carla; Mammi, Stefano
Citation:

Citation: Bellanda, Massimo; Peggion, Evaristo; Otting, Gottfried; Weigelt, Johan; Perdona, Elisabetta; Domenici, Enrico; Marchioro, Carla; Mammi, Stefano. "Letter to the Editor: backbone 1H, 13C, 15N resonance assignments of the N-terminal 24 kDa fragment of the gyrase B subunit from E. coli "  J. Biomol. NMR 22, 369-370 (2002).
PubMed: 12018484

Assembly members:

Assembly members:
24 kDa N-terminal fragment of DNA Gyrase, polymer, 220 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pAM24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
24 kDa N-terminal fragment of DNA Gyrase: MSNSYDSSSIKVLKGLDAVR KRPGMYIGDTDDGTGLHHMV FEVVDNAIDEALAGHCKEII VTIHADNSVSVQDDGRGIPT GIHPEEGVSAAEVIMTVLHA GGKFDDNSYKVSGGLHGVGV SVVNALSQKLELVIQREGKI HRQIYEHGVPQAPLAVTGET EKTGTMVRFWPSLETFTNVT EFEYEILAKRLRELSFLNSG VSIRLRDKRDGKEDHFHYEG

Data sets:
Data typeCount
1H chemical shifts403
13C chemical shifts551
15N chemical shifts196

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GyraseB p241

Entities:

Entity 1, GyraseB p24 220 residues - Formula weight is not available

1   METSERASNSERTYRASPSERSERSERILE
2   LYSVALLEULYSGLYLEUASPALAVALARG
3   LYSARGPROGLYMETTYRILEGLYASPTHR
4   ASPASPGLYTHRGLYLEUHISHISMETVAL
5   PHEGLUVALVALASPASNALAILEASPGLU
6   ALALEUALAGLYHISCYSLYSGLUILEILE
7   VALTHRILEHISALAASPASNSERVALSER
8   VALGLNASPASPGLYARGGLYILEPROTHR
9   GLYILEHISPROGLUGLUGLYVALSERALA
10   ALAGLUVALILEMETTHRVALLEUHISALA
11   GLYGLYLYSPHEASPASPASNSERTYRLYS
12   VALSERGLYGLYLEUHISGLYVALGLYVAL
13   SERVALVALASNALALEUSERGLNLYSLEU
14   GLULEUVALILEGLNARGGLUGLYLYSILE
15   HISARGGLNILETYRGLUHISGLYVALPRO
16   GLNALAPROLEUALAVALTHRGLYGLUTHR
17   GLULYSTHRGLYTHRMETVALARGPHETRP
18   PROSERLEUGLUTHRPHETHRASNVALTHR
19   GLUPHEGLUTYRGLUILELEUALALYSARG
20   LEUARGGLULEUSERPHELEUASNSERGLY
21   VALSERILEARGLEUARGASPLYSARGASP
22   GLYLYSGLUASPHISPHEHISTYRGLUGLY

Related Database Links:

PDB 1AJ6 1EI1 1KZN 3G7E 4DUH 4HYP 4KFG 4PRV 4PRX 4PU9
DBJ BAA20341 BAB38057 BAC06612 BAC06613 BAC06614
EMBL CAA27871 CAP78158 CAQ34043 CAQ91428 CAR00672
GB AAA62050 AAG58896 AAN45208 AAN83054 AAP18989
PIR D86054
REF NP_290332 NP_312661 NP_709501 NP_756480 NP_839178
SP P0AES6 P0AES7 P0AES8
AlphaFold P0AES6 P0AES7 P0AES8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks