BMRB Entry 5772

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5772

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Title:
Backbone 1H, 13C and 15N chemical shifts of CBM13 from Streptomyces lividans
Deposition date:
2003-03-14
Original release date:
2003-05-22
Authors:
Schaerpf, Manuela; McIntosh, Lawrence
Citation:

Citation: Scharpf, Manuela; Connelly, G.; Lee, G.; Boraston, A.; Warren, R.; McIntosh, Lawrence. "Site-specific Characterization of the Association of Xylooligosaccharides with the CBM13 Lectin-like Xylan Binding Domain from Streptomyces lividans Xylanase 10A by NMR Spectroscopy "  Biochemistry 41, 4255-4263 (2002).
PubMed: 11914071

Assembly members:

Assembly members:
family 13 carbohydrate binding modul, polymer, 142 residues, 15035.3 Da.

Natural source:

Natural source:   Common Name: Streptomyces lividans   Taxonomy ID: 1916   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces lividans

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
family 13 carbohydrate binding modul: HHHHHHIEGRASEPPADGGQ IKGVGSGRCLDVPDASTSDG TQLQLWDCHSGTNQQWAATD AGELRVYGDKCLDAAGTSNG SKVQIYSCWGGDNQKWRLNS DGSVVGVQSGLCLDAVGNGT ANGTLIQLYTCSNGSNQRWT RT

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts537
13C chemical shifts481
15N chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CBM131

Entities:

Entity 1, CBM13 142 residues - 15035.3 Da.

1   HISHISHISHISHISHISILEGLUGLYARG
2   ALASERGLUPROPROALAASPGLYGLYGLN
3   ILELYSGLYVALGLYSERGLYARGCYSLEU
4   ASPVALPROASPALASERTHRSERASPGLY
5   THRGLNLEUGLNLEUTRPASPCYSHISSER
6   GLYTHRASNGLNGLNTRPALAALATHRASP
7   ALAGLYGLULEUARGVALTYRGLYASPLYS
8   CYSLEUASPALAALAGLYTHRSERASNGLY
9   SERLYSVALGLNILETYRSERCYSTRPGLY
10   GLYASPASNGLNLYSTRPARGLEUASNSER
11   ASPGLYSERVALVALGLYVALGLNSERGLY
12   LEUCYSLEUASPALAVALGLYASNGLYTHR
13   ALAASNGLYTHRLEUILEGLNLEUTYRTHR
14   CYSSERASNGLYSERASNGLNARGTRPTHR
15   ARGTHR

Related Database Links:

PDB 1KNL 1MC9
EMBL CAD55241
GB AAC26525 AIJ12797 EFD66158 EOY50917
REF NP_733679 WP_003973090 WP_011030540
SP P26514
AlphaFold P26514

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks