BMRB Entry 6362

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PDB ID: 1xjs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6362
MolProbity Validation Chart

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Title:
Solution structure of Iron-Sulfur cluster assembly protein IscU from Bacillus subtilis, with Zinc bound at the active site. Northeast Structural Genomics Consortium Target SR17
Deposition date:
2004-10-27
Original release date:
2005-11-07
Authors:
Kornhaber, G.; Swapna, G.; Ramelot, T.; Cort, J.; Kennedy, M.; Montelione, G.
Citation:

Citation: Ramelot, T.; Cort, J.; Goldsmith-Fischman, S.; Kornhaber, G.; Xiao, R.; Shastry, R.; Acton, T.; Honig, B.; Montelione, G.; Kennedy, M.. "Solution NMR structure of the iron-sulfur cluster assembly protein U (IscU) with zinc bound at the active site"  J. Mol. Biol. 344, 567-583 (2004).
PubMed: 15522305

Assembly members:

Assembly members:
NifU-like protein, polymer, 147 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NifU-like protein: MSFNANLDTLYRQVIMDHYK NPRNKGVLNDSIVVDMNNPT CGDRIRLTMKLDGDIVEDAK FEGEGCSISMASASMMTQAI KGKDIETALSMSKIFSDMMQ GKEYDDSIDLGDIEALQGVS KFPARIKCATLSWKALEKGV AKEEGGN

Data sets:
Data typeCount
1H chemical shifts305
13C chemical shifts316
15N chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NifU-like protein1
2ZINC ION2

Entities:

Entity 1, NifU-like protein 147 residues - Formula weight is not available

1   METSERPHEASNALAASNLEUASPTHRLEU
2   TYRARGGLNVALILEMETASPHISTYRLYS
3   ASNPROARGASNLYSGLYVALLEUASNASP
4   SERILEVALVALASPMETASNASNPROTHR
5   CYSGLYASPARGILEARGLEUTHRMETLYS
6   LEUASPGLYASPILEVALGLUASPALALYS
7   PHEGLUGLYGLUGLYCYSSERILESERMET
8   ALASERALASERMETMETTHRGLNALAILE
9   LYSGLYLYSASPILEGLUTHRALALEUSER
10   METSERLYSILEPHESERASPMETMETGLN
11   GLYLYSGLUTYRASPASPSERILEASPLEU
12   GLYASPILEGLUALALEUGLNGLYVALSER
13   LYSPHEPROALAARGILELYSCYSALATHR
14   LEUSERTRPLYSALALEUGLULYSGLYVAL
15   ALALYSGLUGLUGLYGLYASN

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 1XJS 2AZH
DBJ BAI86813 BAM55339 BAM59352 GAK81939
EMBL CAB15257 CCU60313 CEI58518 CEJ78923
GB ADM39228 ADV94073 AEP88144 AEP92288 AFI29836
REF NP_391147 WP_003222809 WP_003228606 WP_010329998 WP_014480785
SP O32163
AlphaFold O32163 O32163

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks