BMRB Entry 6692

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PDB ID: 2bun
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6692
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments for AppA 5-125
Deposition date:
2005-06-16
Original release date:
2007-02-05
Authors:
Grinstead, Jeffrey; Hsu, Shang-Te; Laan, Wouter; Bonvin, Alexandre; Hellingwerf, Klaas; Boelens, Rolf; Kaptein, Rob
Citation:

Citation: Grinstead, Jeffrey; Hsu, Shang-Te; Laan, Wouter; Bonvin, Alexandre; Hellingwerf, Klaas; Boelens, Rolf; Kaptein, Robert. "The Solution Structure of the AppA BLUF Domain: Insight into the Mechanism of Light-Induced Signaling"  Chem. Biochem. 7, 187-193 (2006).
PubMed: 16323221

Assembly members:

Assembly members:
AppA, polymer, 121 residues, Formula weight is not available
FAD, non-polymer, 785.550 Da.

Natural source:

Natural source:   Common Name: Rhodobacter sphaeroides   Taxonomy ID: 1063   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Rhodobacter sphaeroides

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AppA: LEADVTMTGSDLVSCCYRSL AAPDLTLRDLLDIVETSQAH NARAQLTGALFYSQGVFFQW LEGHPAAVAEVMSHIQRDRR HSNVEILAEESIAKRRFAGW HMQLSCSEADMRSLGLAESR Q

Data sets:
Data typeCount
13C chemical shifts349
15N chemical shifts128
1H chemical shifts798

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1AppA1
2FAD2

Entities:

Entity 1, AppA 121 residues - Formula weight is not available

1   LEUGLUALAASPVALTHRMETTHRGLYSER
2   ASPLEUVALSERCYSCYSTYRARGSERLEU
3   ALAALAPROASPLEUTHRLEUARGASPLEU
4   LEUASPILEVALGLUTHRSERGLNALAHIS
5   ASNALAARGALAGLNLEUTHRGLYALALEU
6   PHETYRSERGLNGLYVALPHEPHEGLNTRP
7   LEUGLUGLYHISPROALAALAVALALAGLU
8   VALMETSERHISILEGLNARGASPARGARG
9   HISSERASNVALGLUILELEUALAGLUGLU
10   SERILEALALYSARGARGPHEALAGLYTRP
11   HISMETGLNLEUSERCYSSERGLUALAASP
12   METARGSERLEUGLYLEUALAGLUSERARG
13   GLN

Entity 2, FAD - C27 H33 N9 O15 P2 - 785.550 Da.

1   FAD

Related Database Links:

PDB 2BUN 4HH1
GB AAA81591 ABA77707 ABN75335 ACM02822 EGJ23059
REF WP_002722303 WP_009564586 WP_011336839 WP_011840246 WP_015921786

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