BMRB Entry 6967

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6967

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Title:
1H and 15N assignment of cytochrome c552 from Thermus thermophilus in the oxidized state
Deposition date:
2006-02-02
Original release date:
2006-04-27
Authors:
Muresanu, Lucia; Pristovsek, Primoz; Loehr, Frank; Maneg, Oliver; Mukrasch, Marco; Rueterjans, Heinz; Ludwig, Bernd; Luecke, Christian
Citation:

Citation: Muresanu, Lucia; Pristovsek, Primoz; Loehr, Frank; Maneg, Oliver; Mukrasch, Marco; Rueterjans, Heinz; Ludwig, Bernd; Luecke, Christian. "The electron transfer complex between cytochrome c552 and the CuA domain of the Thermus thermophilus ba3 oxidase - a combined NMR and computational approach"  J. Biol. Chem. 281, 14503-14513 (2006).
PubMed: 16554303

Assembly members:

Assembly members:
cytochrome c552, polymer, 133 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cytochrome c552: MAQADGAKIYAQCAGCHQQN GQGIPGAFPPLAGHVAEILA KEGGREYLILVLLYGLQGQI EVKGMKYNGVMSSFAQLKDE EIAAVLNHIATAWGDAKKVK GFKPFTAEEVKKLRAKKLTP QQVLTERKKLGLK

Data sets:
Data typeCount
15N chemical shifts144
1H chemical shifts969

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1cyt c5521
2heme c2

Entities:

Entity 1, cyt c552 133 residues - Formula weight is not available

The first 2 residues (Met1 and Ala2) are recombinant artifacts.

1   METALAGLNALAASPGLYALALYSILETYR
2   ALAGLNCYSALAGLYCYSHISGLNGLNASN
3   GLYGLNGLYILEPROGLYALAPHEPROPRO
4   LEUALAGLYHISVALALAGLUILELEUALA
5   LYSGLUGLYGLYARGGLUTYRLEUILELEU
6   VALLEULEUTYRGLYLEUGLNGLYGLNILE
7   GLUVALLYSGLYMETLYSTYRASNGLYVAL
8   METSERSERPHEALAGLNLEULYSASPGLU
9   GLUILEALAALAVALLEUASNHISILEALA
10   THRALATRPGLYASPALALYSLYSVALLYS
11   GLYPHELYSPROPHETHRALAGLUGLUVAL
12   LYSLYSLEUARGALALYSLYSLEUTHRPRO
13   GLNGLNVALLEUTHRGLUARGLYSLYSLEU
14   GLYLEULYS

Entity 2, heme c - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

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