BMRB Entry 7023
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7023
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Citation: Golovanov, Alexander; Hautbergue, Guillaume; Wilson, Stuart; Lian, Lu-Yun. "Assignment of 1H, 13C, and 15N resonances for the REF2-I mRNA export factor" J. Biomol. NMR 36, 41-41 (2006).
PubMed: 16738786
Assembly members:
REF2-I residues 1-155, polymer, 177 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Vector: PET24B
Entity Sequences (FASTA):
REF2-I residues 1-155: MASMTGGQQMGRDPMADKMD
MSLDDIIKLNRNQRRVNRGG
GPRRNRPAIARGGRNRPAPY
SRPKPLPDKWQHDLFDSGCG
GGEGVETGAKLLVSNLDFGV
SDADIQELFAEFGTLKKAAV
DYDRSGRSLGTADVHFERRA
DALKAMKQYKGVPLDGRPMD
IQLVASQIDLEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 576 |
| 15N chemical shifts | 171 |
| 1H chemical shifts | 1068 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chain 1 | 1 |
Entities:
Entity 1, chain 1 177 residues - Formula weight is not available
14-residue N-terminal T7 tag (MASMTGGQQMGRDP) was added to the sequence of protein. C-terminal His6-tag with linker region is also added (LEHHHHHH); signals from five out of six histidins are invisible in the spectra and hence are not assigned.
| 1 | MET | ALA | SER | MET | THR | GLY | GLY | GLN | GLN | MET | ||||
| 2 | GLY | ARG | ASP | PRO | MET | ALA | ASP | LYS | MET | ASP | ||||
| 3 | MET | SER | LEU | ASP | ASP | ILE | ILE | LYS | LEU | ASN | ||||
| 4 | ARG | ASN | GLN | ARG | ARG | VAL | ASN | ARG | GLY | GLY | ||||
| 5 | GLY | PRO | ARG | ARG | ASN | ARG | PRO | ALA | ILE | ALA | ||||
| 6 | ARG | GLY | GLY | ARG | ASN | ARG | PRO | ALA | PRO | TYR | ||||
| 7 | SER | ARG | PRO | LYS | PRO | LEU | PRO | ASP | LYS | TRP | ||||
| 8 | GLN | HIS | ASP | LEU | PHE | ASP | SER | GLY | CYS | GLY | ||||
| 9 | GLY | GLY | GLU | GLY | VAL | GLU | THR | GLY | ALA | LYS | ||||
| 10 | LEU | LEU | VAL | SER | ASN | LEU | ASP | PHE | GLY | VAL | ||||
| 11 | SER | ASP | ALA | ASP | ILE | GLN | GLU | LEU | PHE | ALA | ||||
| 12 | GLU | PHE | GLY | THR | LEU | LYS | LYS | ALA | ALA | VAL | ||||
| 13 | ASP | TYR | ASP | ARG | SER | GLY | ARG | SER | LEU | GLY | ||||
| 14 | THR | ALA | ASP | VAL | HIS | PHE | GLU | ARG | ARG | ALA | ||||
| 15 | ASP | ALA | LEU | LYS | ALA | MET | LYS | GLN | TYR | LYS | ||||
| 16 | GLY | VAL | PRO | LEU | ASP | GLY | ARG | PRO | MET | ASP | ||||
| 17 | ILE | GLN | LEU | VAL | ALA | SER | GLN | ILE | ASP | LEU | ||||
| 18 | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Related Database Links:
| PDB | 2F3J 2YKA |
| DBJ | BAC33282 |
| EMBL | CAB76384 |
| GB | AAH99405 ADJ67482 EDL39077 |
| REF | NP_062357 |
| SP | Q9JJW6 |
| AlphaFold | Q9JJW6 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks