BMRB Entry 7218

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7218

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Title:
1H, 13C and 15N assignment of the Master-Rep protein nuclease domain from the Faba Bean Necrotic Yellows Virus
Deposition date:
2006-07-10
Original release date:
2006-10-30
Authors:
Vega-Rocha, Susana; Campos-Olivas, Ramon
Citation:

Citation: Vega-Rocha, Susana; Gronenborn, A.; Gronenborn, B.; Campos-Olivas, Ramon. "(1)H, (13)C, and (15)N NMR assignment of the master Rep protein nuclease domain from the Nanovirus FBNYV"  J. Biomol. NMR 38, 169-169 (2007).
PubMed: 17043745

Assembly members:

Assembly members:
N-terminal DNA binding domain of M-Rep protein from the nanovirus FBNYV, polymer, 138 residues, 15866.0 Da.

Natural source:

Natural source:   Common Name: Faba Bean Necrotic Yellows Virus   Taxonomy ID: 59817   Superkingdom: Viruses   Kingdom: Viridiplantae   Genus/species: Nanovirus Faba Bean Necrotic Yellows Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-terminal DNA binding domain of M-Rep protein from the nanovirus FBNYV: GSSHHHHHHSSGLEVLFQGP HMARQVICWCFTLNNPLSPL SLHDSMKYLVYQTEQGEAGN IHFQGYIEMKKRTSLAGMKK LIPGAHFEKRRGTQGEARAY SMKEDTRLEGPWEYGEFVPT IEDKLREVMNDMKITGKR

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts127
1H chemical shifts559
coupling constants74

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 138 residues - 15866.0 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUGLUVALLEUPHEGLNGLYPRO
3   HISMETALAARGGLNVALILECYSTRPCYS
4   PHETHRLEUASNASNPROLEUSERPROLEU
5   SERLEUHISASPSERMETLYSTYRLEUVAL
6   TYRGLNTHRGLUGLNGLYGLUALAGLYASN
7   ILEHISPHEGLNGLYTYRILEGLUMETLYS
8   LYSARGTHRSERLEUALAGLYMETLYSLYS
9   LEUILEPROGLYALAHISPHEGLULYSARG
10   ARGGLYTHRGLNGLYGLUALAARGALATYR
11   SERMETLYSGLUASPTHRARGLEUGLUGLY
12   PROTRPGLUTYRGLYGLUPHEVALPROTHR
13   ILEGLUASPLYSLEUARGGLUVALMETASN
14   ASPMETLYSILETHRGLYLYSARG

Related Database Links:

BMRB 7112
PDB 2HWT
EMBL CAA72209 CAB44020
GB ACX50503
REF NP_619567
SP O39828 Q9WIJ5
AlphaFold O39828 Q9WIJ5

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SPARKY: Backbone or all simulated peaks