BMRB Entry 10087
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10087
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NMR-STAR v3 text file.
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Title: Solution structure of C-terminal ubiquitin like domain of human 2'-5'-oligoadenylate synthetase-like protain (p59 OASL)
Deposition date: 2007-01-24 Original release date: 2008-08-14
Authors: Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of C-terminal ubiquitin like domain of human 2'-5'-oligoadenylate synthetase-like protain (p59 OASL)" .
Assembly members:
ubiquitin like domain, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Vector: P040301-13
Entity Sequences (FASTA):
ubiquitin like domain: GSSGSSGIQVFVKNPDGGSY
AYAINPNSFILGLKQQIEDQ
QGLPKKQQQLEFQGQVLQDW
LGLGIYGIQDSDTLILSKKK
GSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 348 |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 550 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 59 kDa 2'-5'-oligoadenylate synthetase like protein | 1 |
Entities:
Entity 1, 59 kDa 2'-5'-oligoadenylate synthetase like protein 87 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ILE | GLN | VAL | ||||
| 2 | PHE | VAL | LYS | ASN | PRO | ASP | GLY | GLY | SER | TYR | ||||
| 3 | ALA | TYR | ALA | ILE | ASN | PRO | ASN | SER | PHE | ILE | ||||
| 4 | LEU | GLY | LEU | LYS | GLN | GLN | ILE | GLU | ASP | GLN | ||||
| 5 | GLN | GLY | LEU | PRO | LYS | LYS | GLN | GLN | GLN | LEU | ||||
| 6 | GLU | PHE | GLN | GLY | GLN | VAL | LEU | GLN | ASP | TRP | ||||
| 7 | LEU | GLY | LEU | GLY | ILE | TYR | GLY | ILE | GLN | ASP | ||||
| 8 | SER | ASP | THR | LEU | ILE | LEU | SER | LYS | LYS | LYS | ||||
| 9 | GLY | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: ubiquitin like domain, [U-13C; U-15N], 1.1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.8994, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts