BMRB Entry 10162

Name: Solution structure of the 23th filamin domain from human Filamin-B
Published: 2009-02-20

Click here to enlarge.

PDB ID: 2eec
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10162
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the 23th filamin domain from human Filamin-B

Deposition date:

2007-12-28

Original release date:

2009-02-20

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 23th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
filamin domain, Filamin 23, polymer, 125 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060619-24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
filamin domain, Filamin 23: GSSGSSGFKVRVGEPGQAGN PALVSAYGTGLEGGTTGIQS EFFINTTRAGPGTLSVTIEG PSKVKMDCQETPEGYKVMYT PMAPGNYLISVKYGGPNHIV GSPFKAKVTGQRLVSPGSAN ETSSI

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	453
15N chemical shifts	100
1H chemical shifts	730

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin-B	1

Entities:

Entity 1, Filamin-B 125 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PHE

LYS

VAL

2

ARG

VAL

GLY

GLU

PRO

GLY

GLN

ALA

GLY

ASN

3

PRO

ALA

LEU

VAL

SER

ALA

TYR

GLY

THR

GLY

4

LEU

GLU

GLY

THR

GLY

ILE

GLN

SER

5

GLU

PHE

ILE

ASN

THR

ARG

ALA

GLY

6

PRO

GLY

THR

LEU

SER

VAL

THR

ILE

GLU

GLY

7

PRO

SER

LYS

VAL

LYS

MET

ASP

CYS

GLN

GLU

8

THR

PRO

GLU

GLY

TYR

LYS

VAL

MET

TYR

THR

9

PRO

MET

ALA

PRO

GLY

ASN

TYR

LEU

ILE

SER

10

VAL

LYS

TYR

GLY

PRO

ASN

HIS

ILE

VAL

11

GLY

SER

PRO

PHE

LYS

ALA

LYS

VAL

THR

GLY

12

GLN

ARG

LEU

VAL

SER

PRO

GLY

SER

ALA

ASN

13

GLU

THR

SER

ILE

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz