BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11145

Title: Solution structure of the CH domain from human EH domain binding protein 1

Deposition date: 2010-03-31 Original release date: 2011-04-01

Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain from human EH domain binding protein 1"  .

Assembly members:
CH domain, polymer, 119 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050725-07

Entity Sequences (FASTA):
CH domain: GSSGSSGPNASQSLLVWCKE VTKNYRGVKITNFTTSWRNG LSFCAILHHFRPDLIDYKSL NPQDIKENNKKAYDGFASIG ISRLLEPSDMVLLAIPDKLT VMTYLYQIRAHFSSGPSSG

Data sets:
Data typeCount
13C chemical shifts520
15N chemical shifts114
1H chemical shifts809

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CH domain1

Entities:

Entity 1, CH domain 119 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROASNALA
2   SERGLNSERLEULEUVALTRPCYSLYSGLU
3   VALTHRLYSASNTYRARGGLYVALLYSILE
4   THRASNPHETHRTHRSERTRPARGASNGLY
5   LEUSERPHECYSALAILELEUHISHISPHE
6   ARGPROASPLEUILEASPTYRLYSSERLEU
7   ASNPROGLNASPILELYSGLUASNASNLYS
8   LYSALATYRASPGLYPHEALASERILEGLY
9   ILESERARGLEULEUGLUPROSERASPMET
10   VALLEULEUALAILEPROASPLYSLEUTHR
11   VALMETTHRTYRLEUTYRGLNILEARGALA
12   HISPHESERSERGLYPROSERSERGLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 0.85 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA74926 BAD32333 BAF85234
EMBL CAD38814
GB AAH67215 AAI68392 AAL24806 AAQ97141 AAS48537
REF NP_001136086 NP_001136087 NP_001136088 NP_001239444 NP_001248495
SP Q69ZW3 Q8NDI1
TPG DAA24644 DAA24645 DAA24646
AlphaFold Q8NDI1 Q69ZW3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts