BMRB Entry 11224
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11224
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Title: Solution structure of the SH3 domain from Phospholipase C, gamma 2
Deposition date: 2010-07-23 Original release date: 2011-08-03
Authors: Qin, X.; Nagashima, T.; Hayahsi, F.; Yokoyama, S.
Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain from Phospholipase C, gamma 2" .
Assembly members:
SH3 domain, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050815-04
Entity Sequences (FASTA):
SH3 domain: GSSGSSGRTVKALYDYKAKR
SDELTFCRGALIHNVSKEPG
GWWKGDYGTRIQQYFPSNYV
EDISGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 284 |
| 15N chemical shifts | 63 |
| 1H chemical shifts | 430 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 69 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ARG | THR | VAL | ||||
| 2 | LYS | ALA | LEU | TYR | ASP | TYR | LYS | ALA | LYS | ARG | ||||
| 3 | SER | ASP | GLU | LEU | THR | PHE | CYS | ARG | GLY | ALA | ||||
| 4 | LEU | ILE | HIS | ASN | VAL | SER | LYS | GLU | PRO | GLY | ||||
| 5 | GLY | TRP | TRP | LYS | GLY | ASP | TYR | GLY | THR | ARG | ||||
| 6 | ILE | GLN | GLN | TYR | PHE | PRO | SER | ASN | TYR | VAL | ||||
| 7 | GLU | ASP | ILE | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, varian - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92151 BAG10628 BAG65485 |
| EMBL | CAA32194 |
| GB | AAA41896 AAA60112 AAH07565 AAH11772 AAH14561 |
| REF | NP_002652 NP_058864 NP_758489 XP_001148089 XP_001502048 |
| SP | P16885 P24135 Q8CIH5 |
| AlphaFold | P24135 P16885 Q8CIH5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts