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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11540
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Costa, Liliana; Marshall, Eleanor; Tesfaye, Mesfin; Silverstein, Kevin; Mori, Masashi; Umetsu, Yoshitaka; Otterbach, Sophie; Papareddy, Ranjith; Dickinson, Hugh; Boutiller, Kim; VandenBosch, Kathryn; Ohki, Shinya; Gutierrez-Marcos, Jose. "Central cell-derived peptides regulate early embryo patterning in flowering plants" Science 344, 168-172 (2014).
PubMed: 24723605
Assembly members:
entity, polymer, 70 residues, 7976.471 Da.
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: TOMATO MOSAIC VIRUS Vector: PBICER8-C0.3-HUIFN-G-SRZ
Entity Sequences (FASTA):
entity: GPEKMEVKEIGRSSKIILPA
CMHETCSGGFSLKNDCWCCL
RLKTKQARCWKEKEFPNAKE
LCFANCPPLE
Data type | Count |
13C chemical shifts | 177 |
15N chemical shifts | 68 |
1H chemical shifts | 421 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity | 1 |
Entity 1, entity 70 residues - 7976.471 Da.
1 | GLY | PRO | GLU | LYS | MET | GLU | VAL | LYS | GLU | ILE | |
2 | GLY | ARG | SER | SER | LYS | ILE | ILE | LEU | PRO | ALA | |
3 | CYS | MET | HIS | GLU | THR | CYS | SER | GLY | GLY | PHE | |
4 | SER | LEU | LYS | ASN | ASP | CYS | TRP | CYS | CYS | LEU | |
5 | ARG | LEU | LYS | THR | LYS | GLN | ALA | ARG | CYS | TRP | |
6 | LYS | GLU | LYS | GLU | PHE | PRO | ASN | ALA | LYS | GLU | |
7 | LEU | CYS | PHE | ALA | ASN | CYS | PRO | PRO | LEU | GLU |
sample_1: entity, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 50 mM; potassium chloride 50 mM; H2O 90%; D2O 10%
sample_2: entity, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 50 mM; potassium chloride 50 mM; D2O 100%
sample_conditions_1: ionic strength: 100 mM; pH: 5.8; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_2 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks