BMRB Entry 11550

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11550

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Active-Site Structure of the Thermophilic Foc-Subunit Ring in Membranes Elucidated by Solid-State NMR

Deposition date:

2014-02-10

Original release date:

2017-08-03

Authors:

Kang, Su-Jin; Yasuto, Todokoro; Akustu, Hideo

Citation:

Citation: Kang, Su-Jin; Yasuto, Todokoro; Ikuko, Yumen; Bo, Shen; Iku, Iwasaki; Toshiharu, Suzuki; Atsushi, Miyagi; Masasuke, Yoshida; Toshimichi, Fujiwara; Akutsu, Hideo. "Active-site structure of the thermophilic foc-subunit ring in membranes elucidated by solid-state NMR" Biophys J. 106, 390-398 (2014).
PubMed: 24461014

Assembly members:

Assembly members:
subunit c of thermophilic FoF1 ATP synthase, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus sp. PS3 Taxonomy ID: 2334 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: wheat germ - cell free Vector: pZA01MCS-SP6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
subunit c of thermophilic FoF1 ATP synthase: MHLGVLAAAIAVGLGALGAG IGNGLIVSRTIEGIARQPEL RPVLQTTMFIGVALVEALPI IGVVFSFIYLGR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list
spectral_peak_list

Data type	Count
13C chemical shifts	19

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Foc-subunit_1	1
2	Foc-subunit_2	1
3	Foc-subunit_3	1
4	Foc-subunit_4	1
5	Foc-subunit_5	1
6	Foc-subunit_6	1
7	Foc-subunit_7	1
8	Foc-subunit_8	1
9	Foc-subunit_9	1
10	Foc-subunit_10	1

Entities:

Entity 1, Foc-subunit_1 72 residues - Formula weight is not available

1

MET

HIS

LEU

GLY

VAL

LEU

ALA

ILE

2

ALA

VAL

GLY

LEU

GLY

ALA

LEU

GLY

ALA

GLY

3

ILE

GLY

ASN

GLY

LEU

ILE

VAL

SER

ARG

THR

4

ILE

GLU

GLY

ILE

ALA

ARG

GLN

PRO

GLU

LEU

5

ARG

PRO

VAL

LEU

GLN

THR

MET

PHE

ILE

6

GLY

VAL

ALA

LEU

VAL

GLU

ALA

LEU

PRO

ILE

7

ILE

GLY

VAL

PHE

SER

PHE

ILE

TYR

LEU

8

GLY

ARG

Samples:

sample_1: thermophilic Foc-subunit ring, [U-100% 13C; U-100% 15N; U-80% 2H], 2.8 mg; D2O 100%; Tris-DCl 10 mM

sample_conditions_1: ionic strength: 0.010 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
DARR	sample_1	solid	sample_conditions_1
DARR	sample_1	solid	sample_conditions_1
Cai+1-(C'Ca) correlation	sample_1	solid	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian infinity-plus 700 MHz
Varian infinity-plus 600 MHz