BMRB Entry 11592

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PDB ID: 2rva
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11592
MolProbity Validation Chart

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Title:
Solution structure of chitosan-binding module 2 derived from chitosanase/glucanase from Paenibacillus sp. IK-5
Deposition date:
2015-05-13
Original release date:
2016-04-04
Authors:
Shinya, Shoko; Nishimura, Shigenori; Fukamizo, Tamo
Citation:

Citation: Shinya, Shoko; Nishimura, Shigenori; Kitaoku, Yoshihito; Ohnuma, Takayuki; Numata, Tomoyuki; Kimoto, Hisashi; Kusaoke, Hideo; Fukamizo, Tamo. "Mechanism of chitosan recognition by CBM32 carbohydrate-binding modules from a Paenibacillus sp. IK-5 chitosanase/glucanase"  Biochem. J. ., .-. (2016).
PubMed: 26936968

Assembly members:

Assembly members:
DD2, polymer, 131 residues, 14081.312 Da.

Natural source:

Natural source:   Common Name: Paenibacillus sp.   Taxonomy ID: 44249   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Paenibacillus sp.

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET Blue-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DD2: NLALNKATATSSIETAGHEG DKAVDGNAATRWASAYGASP QWIYINLGSTQSISRVKLNW EDAYATAYSIQVSNDSGSTP TNWTTVYSTTTGDGAIDDIT FAATNAKFVRVYATTRATAY GYSLWEFEVYG

Data sets:
Data typeCount
13C chemical shifts469
15N chemical shifts137
1H chemical shifts671

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 131 residues - 14081.312 Da.

1   ASNLEUALALEUASNLYSALATHRALATHR
2   SERSERILEGLUTHRALAGLYHISGLUGLY
3   ASPLYSALAVALASPGLYASNALAALATHR
4   ARGTRPALASERALATYRGLYALASERPRO
5   GLNTRPILETYRILEASNLEUGLYSERTHR
6   GLNSERILESERARGVALLYSLEUASNTRP
7   GLUASPALATYRALATHRALATYRSERILE
8   GLNVALSERASNASPSERGLYSERTHRPRO
9   THRASNTRPTHRTHRVALTYRSERTHRTHR
10   THRGLYASPGLYALAILEASPASPILETHR
11   PHEALAALATHRASNALALYSPHEVALARG
12   VALTYRALATHRTHRARGALATHRALATYR
13   GLYTYRSERLEUTRPGLUPHEGLUVALTYR
14   GLY

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