BMRB Entry 15136
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15136
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Endosulfine alpha PubMed: 19636856
Deposition date: 2007-02-15 Original release date: 2008-01-09
Authors: Boettcher, John; Hartman, Kevin; Ladror, Daniel; Qi, Zhi; Woods, Wendy; Rienstra, Chad; George, Julia
Citation: Boettcher, John; Hartman, Kevin; Ladror, Daniel; Qi, Zhi; Woods, Wendy; George, Julia; Rienstra, Chad. "(1)H, (13)C, and (15)N resonance assignment of the cAMP-regulated phosphoprotein endosulfine-alpha in free and micelle-bound states" Biomol. NMR Assignments 1, 167-169 (2007).
Assembly members:
ENSA, polymer, 121 residues, 13.389 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: ????
Entity Sequences (FASTA):
ENSA: MSQKQEEENPAEETGEEKQD
TQEKEGILPERAEEAKLKAK
YPSLGQKPGGSDFLMKRLQK
GQKYFDSGDYNMAKAKMKNK
QLPSAGPDKNLVTGDHIPTP
QDLPQRKSSLVTSKLAGGQV
E
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 304 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ENSA, chain 1 | 1 |
| 2 | ENSA, chain 2 | 1 |
Entities:
Entity 1, ENSA, chain 1 121 residues - 13.389 Da.
| 1 | MET | SER | GLN | LYS | GLN | GLU | GLU | GLU | ASN | PRO | ||||
| 2 | ALA | GLU | GLU | THR | GLY | GLU | GLU | LYS | GLN | ASP | ||||
| 3 | THR | GLN | GLU | LYS | GLU | GLY | ILE | LEU | PRO | GLU | ||||
| 4 | ARG | ALA | GLU | GLU | ALA | LYS | LEU | LYS | ALA | LYS | ||||
| 5 | TYR | PRO | SER | LEU | GLY | GLN | LYS | PRO | GLY | GLY | ||||
| 6 | SER | ASP | PHE | LEU | MET | LYS | ARG | LEU | GLN | LYS | ||||
| 7 | GLY | GLN | LYS | TYR | PHE | ASP | SER | GLY | ASP | TYR | ||||
| 8 | ASN | MET | ALA | LYS | ALA | LYS | MET | LYS | ASN | LYS | ||||
| 9 | GLN | LEU | PRO | SER | ALA | GLY | PRO | ASP | LYS | ASN | ||||
| 10 | LEU | VAL | THR | GLY | ASP | HIS | ILE | PRO | THR | PRO | ||||
| 11 | GLN | ASP | LEU | PRO | GLN | ARG | LYS | SER | SER | LEU | ||||
| 12 | VAL | THR | SER | LYS | LEU | ALA | GLY | GLY | GLN | VAL | ||||
| 13 | GLU |
Samples:
sample_1: sodium phosphate 50 mM; sodium azide 0.02%; ENSA, [U-100% 13C; U-100% 15N], 0.5 mM
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNN | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
SPARKY, Goddard - data analysis, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 15135 |
| DBJ | BAB24433 BAB27552 BAB31584 BAE29049 BAE36298 |
| EMBL | CAA06798 CAA06799 CAA06800 CAA06801 CAA65196 |
| GB | AAD32454 AAF80340 AAF80341 AAH00436 AAH04461 |
| REF | NP_001021383 NP_001029146 NP_001300934 NP_004427 NP_062507 |
| SP | O43768 P60840 P60841 P68210 P68211 |
| TPG | DAA31642 |
| AlphaFold | P68211 O43768 P60840 P60841 P68210 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts