BMRB Entry 15138
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15138
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Title: 13C and 15N resonance assignments for the major coat protein in Pf1 filamentous bacteriophage PubMed: 17279748
Deposition date: 2007-02-21 Original release date: 2007-03-06
Authors: Goldbourt, Amir; Gross, Benjamin; Day, Loren; McDermott, Ann
Citation: Goldbourt, Amir; Gross, Benjamin; Day, Loren; McDermott, Ann. "Filamentous phage studied by magic-angle spinning NMR: resonance assignment and secondary structure of the coat protein in Pf1." J. Am. Chem. Soc. 129, 2338-2344 (2007).
Assembly members:
Pf1_phage_coat_protein, polymer, 46 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: Purified from host after deliberate infection Host organism: Pseudomonas aeruginosa
Entity Sequences (FASTA):
Pf1_phage_coat_protein: GVIDTSAVESAITDGQGDMK
AIGGYIVGALVILAVAGLIY
SMLRKA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 196 |
| 15N chemical shifts | 49 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Coat protein | 1 |
Entities:
Entity 1, Coat protein 46 residues - Formula weight is not available
This is the major coat protein of Pf1 filamentous bacteriophage. Also referred to as gene-8 protein product, g8p.
| 1 | GLY | VAL | ILE | ASP | THR | SER | ALA | VAL | GLU | SER | ||||
| 2 | ALA | ILE | THR | ASP | GLY | GLN | GLY | ASP | MET | LYS | ||||
| 3 | ALA | ILE | GLY | GLY | TYR | ILE | VAL | GLY | ALA | LEU | ||||
| 4 | VAL | ILE | LEU | ALA | VAL | ALA | GLY | LEU | ILE | TYR | ||||
| 5 | SER | MET | LEU | ARG | LYS | ALA |
Samples:
Pf1_U: Pf1 phage, [U-13C; U-15N], 7 – 10 mg
Pf1_NCO: Pf1 phage, [U-15N; 13C C1-Glucose], 7 – 10 mg
sample_conditions_1: pH: 8.4; temperature: 297.5 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D NCACX | Pf1_U | Precipitated solid | sample_conditions_1 |
| 3D NCOCX | Pf1_U | Precipitated solid | sample_conditions_1 |
| 2D CC DARR | Pf1_U | Precipitated solid | sample_conditions_1 |
| 3D NCOCX | Pf1_NCO | Precipitated solid | sample_conditions_1 |
| 2D NN PDSD | Pf1_NCO | Precipitated solid | sample_conditions_1 |
| 3D NCACX | Pf1_U | Precipitated solid | sample_conditions_1 |
| 3D NCOCX | Pf1_U | Precipitated solid | sample_conditions_1 |
| 2D CC DARR | Pf1_U | Precipitated solid | sample_conditions_1 |
| 2D CC DARR | Pf1_U | Precipitated solid | sample_conditions_1 |
Software:
SPARKY v3.110, Goddard - chemical shift assignment, peak picking
NMRPipe v2.3 Rev 2004.184.22.03, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker DRX 750 MHz
- Varian InfinityPlus 600 MHz
- Varian InfinityPlus 400 MHz