BMRB Entry 15258

Assembly:

Entities:

Entity 1, subunit 1 166 residues - 18557.6 Da.

8 residues C-terminal His tag (LEHHHHHH)

Samples:

NC_sample: VP2129, [U-13C; U-15N], 1.4 ± 0.1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.25 mM; ammonium acetate 20 ± 1 mM; DTT 10 ± 0.5 mM; sodium azide 0.02 ± 0.001 %; H2O 95%; D2O 5%

NC5_sample: VP2129, [U-5% 13C; U-100% 15N], 1.4 ± 0.1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.25 mM; ammonium acetate 20 ± 1 mM; DTT 10 ± 0.5 mM; sodium azide 0.02 ± 0.001 %; H2O 95%; D2O 5%

NC_in_D2O: VP2129, [U-13C; U-15N], 1.4 ± 0.1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.25 mM; ammonium acetate 20 ± 1 mM; DTT 10 ± 0.5 mM; sodium azide 0.02 ± 0.001 %; D2O 100%

NC50_sample_in_D2O: VP2129, [U-13C; U-15N], 0.4 ± 0.1 mM; sodium chloride 100 ± 5 mM; calcium chloride 5 ± 0.25 mM; ammonium acetate 20 ± 1 mM; DTT 10 ± 0.5 mM; sodium azide 0.02 ± 0.001 %; VP2129 0.4 ± 0.1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.1 M; pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

Software:

NMRPipe vnmrpipe_linux, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMR, Varian - collection

AutoStruct v2.1.1, Huang, Tejero, Powers and Montelione - data analysis

X-PLOR NIH v2.15.0, Schwieters, Kuszewski, Tjandra and Clore - structure solution

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

SPARKY v3.1, Goddard - data analysis

NMR spectrometers:

• Varian INOVA 500 MHz
• Varian INOVA 600 MHz
• Varian INOVA 750 MHz