BMRB Entry 15409
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15409
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the cytoplasmic domain of the T cell receptor zeta chain PubMed: 18311971
Deposition date: 2007-07-30 Original release date: 2008-03-14
Authors: Jaravine, Victor; Zhuravleva, Anastasia; Permi, Perttu; Ibraghimov, Ilgis; Orekhov, Vladislav
Citation: Jaravine, Victor; Zhuravleva, Anastasia; Permi, Perttu; Ibraghimov, Ilgis; Orekhov, Vladislav. "Hyperdimensional NMR Spectroscopy with Nonlinear Sampling." J. Am. Chem. Soc. 130, 3927-3936 (2008).
Assembly members:
T-cell_receptor_zeta-chain, polymer, 115 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a(+)
Entity Sequences (FASTA):
T-cell_receptor_zeta-chain: GSLRVKFSRSADAPAYQQGQ
NQLYNELNLGRREEYDVLDK
RRGRDPEMGGKPRRKNPQEG
LYNELQKDKMAEAYSEIGMK
GERRRGKGHDGLYQGLSTAT
KDTYDALHMQALPPR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 321 |
| 15N chemical shifts | 104 |
| 1H chemical shifts | 104 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 115 residues - Formula weight is not available
| 1 | GLY | SER | LEU | ARG | VAL | LYS | PHE | SER | ARG | SER | ||||
| 2 | ALA | ASP | ALA | PRO | ALA | TYR | GLN | GLN | GLY | GLN | ||||
| 3 | ASN | GLN | LEU | TYR | ASN | GLU | LEU | ASN | LEU | GLY | ||||
| 4 | ARG | ARG | GLU | GLU | TYR | ASP | VAL | LEU | ASP | LYS | ||||
| 5 | ARG | ARG | GLY | ARG | ASP | PRO | GLU | MET | GLY | GLY | ||||
| 6 | LYS | PRO | ARG | ARG | LYS | ASN | PRO | GLN | GLU | GLY | ||||
| 7 | LEU | TYR | ASN | GLU | LEU | GLN | LYS | ASP | LYS | MET | ||||
| 8 | ALA | GLU | ALA | TYR | SER | GLU | ILE | GLY | MET | LYS | ||||
| 9 | GLY | GLU | ARG | ARG | ARG | GLY | LYS | GLY | HIS | ASP | ||||
| 10 | GLY | LEU | TYR | GLN | GLY | LEU | SER | THR | ALA | THR | ||||
| 11 | LYS | ASP | THR | TYR | ASP | ALA | LEU | HIS | MET | GLN | ||||
| 12 | ALA | LEU | PRO | PRO | ARG |
Samples:
sample_1: T-cell receptor zeta-chain, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%; Phosphate buffer 20 mM
sample_conditions_1: ionic strength: 0.02 M; pH: 6.7; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D iHNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D iHN(CA)CB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.6, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing
AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment
MDD v1.3, (MDDnmr) V.Orekhov, V.Jaravine - data analysis, processing
NMR spectrometers:
- Varian INOVA 900 MHz
Related Database Links:
| DBJ | BAC87407 BAD97045 BAG36664 BAJ20574 |
| GB | AAA60394 AAD34640 AAF34793 AAH25703 AAY57330 |
| REF | NP_000725 NP_932170 XP_001174731 XP_003258862 XP_003824647 |
| SP | P20963 Q9XSJ9 |
| AlphaFold | Q9XSJ9 P20963 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts