BMRB Entry 15509
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15509
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kaustov, Lilia; Liao, Jack; Lemak, Sasha; Duan, Shili; Muhandiram, Ranjith; Karra, Murthy; Srisailam, Sampath; Dhe-Paganon, Sirano; Arrowsmith, Cheryl. "NMR Solution Structure of PARC CPH Domain" .
Assembly members:
PARC, polymer, 105 residues, 15094.872 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
PARC: GSHMRSEFSSRGGYGEYVQQ
TLQPGMRVRMLDDYEEISAG
DEGEFRQSNNGIPPVQVFWQ
STGRTYWVHWHMLEILGPEE
ATEDKASAAVEKGAGATVLG
TAFPS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 372 |
| 15N chemical shifts | 105 |
| 1H chemical shifts | 639 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 105 residues - 15094.872 Da.
Residues 1-4 represent a residual tag after His-tag cleavage
| 1 | GLY | SER | HIS | MET | ARG | SER | GLU | PHE | SER | SER | ||||
| 2 | ARG | GLY | GLY | TYR | GLY | GLU | TYR | VAL | GLN | GLN | ||||
| 3 | THR | LEU | GLN | PRO | GLY | MET | ARG | VAL | ARG | MET | ||||
| 4 | LEU | ASP | ASP | TYR | GLU | GLU | ILE | SER | ALA | GLY | ||||
| 5 | ASP | GLU | GLY | GLU | PHE | ARG | GLN | SER | ASN | ASN | ||||
| 6 | GLY | ILE | PRO | PRO | VAL | GLN | VAL | PHE | TRP | GLN | ||||
| 7 | SER | THR | GLY | ARG | THR | TYR | TRP | VAL | HIS | TRP | ||||
| 8 | HIS | MET | LEU | GLU | ILE | LEU | GLY | PRO | GLU | GLU | ||||
| 9 | ALA | THR | GLU | ASP | LYS | ALA | SER | ALA | ALA | VAL | ||||
| 10 | GLU | LYS | GLY | ALA | GLY | ALA | THR | VAL | LEU | GLY | ||||
| 11 | THR | ALA | PHE | PRO | SER |
Samples:
PARC_sample: TRIS, [U-13C; U-15N], 25 mM; sodium chloride 250 mM; H2O 90%; PMSF 0.5 mM; Benzamidine 1 mM; D2O 10%; H2O 55 mM
PARC_cond: ionic strength: 0.25 M; pH: 7.4; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | PARC_sample | isotropic | PARC_cond |
| 2D 1H-13C HSQC | PARC_sample | isotropic | PARC_cond |
| 3D HNCACB | PARC_sample | isotropic | PARC_cond |
| 3D CBCA(CO)NH | PARC_sample | isotropic | PARC_cond |
| 3D Cc(CO)NH-TOCSY | PARC_sample | isotropic | PARC_cond |
| 3D 1H-15N NOESY | PARC_sample | isotropic | PARC_cond |
| 3D 1H-13C NOESY | PARC_sample | isotropic | PARC_cond |
| 3D HCCH-TOCSY | PARC_sample | isotropic | PARC_cond |
| 3D HNCO | PARC_sample | isotropic | PARC_cond |
| 3D HBHA(CO)NH | PARC_sample | isotropic | PARC_cond |
| Hc(CO)NH-TOCSY | PARC_sample | isotropic | PARC_cond |
Software:
CYANA, Brunger, Adams, Clore, Gros, Nilges and Read, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, peak picking, processing, refinement, structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
- Varian INOVA 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAI45256 |
| EMBL | CAC85756 CAH18328 CAH18696 |
| GB | AAH28159 AAI48598 AAN61516 EAX04163 EAX04164 |
| REF | NP_055904 XP_002746613 XP_002808431 XP_003833312 XP_003897675 |
| SP | Q8IWT3 |
| AlphaFold | Q8IWT3 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks